Application | Comment | Organism |
---|---|---|
synthesis | upon codon optimization for expression in Pichia pastoris, the expression level increases to 5.7 g/l in a fermenter system | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
3,4-dinitrophenyl beta-D-xylopyranoside | pH 4.0, 40°C | Aspergillus niger | |
0.66 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.0, 40°C | Aspergillus niger | |
4.01 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.0, 40°C | Aspergillus niger | |
4.54 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 4.0, 40°C | Aspergillus niger | |
30.8 | - |
xylobiose | pH 4.0, 40°C | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A0A023J5W7 | - |
- |
Aspergillus niger ASKU28 | A0A023J5W7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus niger | 3,4-dinitrophenol + D-xylopyranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger ASKU28 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger ASKU28 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + D-xylopyranose | - |
? | |
beechwood xylan + H2O | - |
Aspergillus niger | ? | - |
? | |
beechwood xylan + H2O | - |
Aspergillus niger ASKU28 | ? | - |
? | |
additional information | the enzyme displays an exo-type activity with retention of the beta-configuration, and a synergistic action with xylanase in hydrolysis of beechwood xylan | Aspergillus niger | ? | - |
? | |
additional information | the enzyme displays an exo-type activity with retention of the beta-configuration, and a synergistic action with xylanase in hydrolysis of beechwood xylan | Aspergillus niger ASKU28 | ? | - |
? | |
xylobiose + H2O | - |
Aspergillus niger | 2 D-xylose | - |
? | |
xylobiose + H2O | - |
Aspergillus niger ASKU28 | 2 D-xylose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.0, 40°C | Aspergillus niger | |
67.8 | - |
xylobiose | pH 4.0, 40°C | Aspergillus niger | |
93.8 | - |
3,4-dinitrophenyl beta-D-xylopyranoside | pH 4.0, 40°C | Aspergillus niger | |
113 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 4.0, 40°C | Aspergillus niger | |
334 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.0, 40°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
xylobiose | pH 4.0, 40°C | Aspergillus niger | |
3.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.0, 40°C | Aspergillus niger | |
24.9 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 4.0, 40°C | Aspergillus niger | |
242 | - |
3,4-dinitrophenyl beta-D-xylopyranoside | pH 4.0, 40°C | Aspergillus niger | |
504 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.0, 40°C | Aspergillus niger |