Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | weak inhibition | Bifidobacterium breve | |
Mn2+ | weak inhibition | Bifidobacterium breve | |
p-chloromercuryphenylsulfonic acid | potent inhibitor | Bifidobacterium breve |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.54 | - |
4-nitrophenyl beta-D-xylopyranoside | in 50 mM phosphate buffer (pH 7.0), at 37°C | Bifidobacterium breve | |
4.76 | - |
ginsenoside Ra2 | in 50 mM phosphate buffer (pH 7.0), at 37°C | Bifidobacterium breve | |
5.56 | - |
ginsenoside Ra1 | in 50 mM phosphate buffer (pH 7.0), at 37°C | Bifidobacterium breve |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE | Bifidobacterium breve |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium breve | B1P195 | - |
- |
Bifidobacterium breve K-110 | B1P195 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, QAE cellulose column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, and Q Sepharose column chromatography | Bifidobacterium breve |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
crude extract, in 50 mM phosphate buffer (pH 7.0), at 37°C | Bifidobacterium breve |
51.82 | - |
1036fold purified enzyme, in 50 mM phosphate buffer (pH 7.0), at 37°C | Bifidobacterium breve |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | 100% activity | Bifidobacterium breve | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 100% activity | Bifidobacterium breve K-110 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
ginsenoside Ra1 + H2O | 5.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside | Bifidobacterium breve | ginsenoside Rb2 + D-xylose | - |
? | |
ginsenoside Ra1 + H2O | 5.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside | Bifidobacterium breve K-110 | ginsenoside Rb2 + D-xylose | - |
? | |
ginsenoside Ra2 + H2O | 4.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside | Bifidobacterium breve | ginsenoside Rc + D-xylose | - |
? | |
ginsenoside Ra2 + H2O | 4.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside | Bifidobacterium breve K-110 | ginsenoside Rc + D-xylose | - |
? | |
additional information | the enzyme does not act on 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, maltose, cellobiose, ginsenoside Rb2, and ginsenoside Rc | Bifidobacterium breve | ? | - |
? | |
additional information | the enzyme does not act on 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, maltose, cellobiose, ginsenoside Rb2, and ginsenoside Rc | Bifidobacterium breve K-110 | ? | - |
? | |
xylan + H2O | 0.4% activity compared to 4-nitrophenyl beta-D-xylopyranoside | Bifidobacterium breve | ? | - |
? | |
xylan + H2O | 0.4% activity compared to 4-nitrophenyl beta-D-xylopyranoside | Bifidobacterium breve K-110 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Bifidobacterium breve |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
at 37°C | Bifidobacterium breve |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bifidobacterium breve | isoelectric focusing | - |
4.2 |