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Literature summary for 3.2.1.37 extracted from

  • Zhou, J.; Bao, L.; Chang, L.; Liu, Z.; You, C.; Lu, H.
    beta-Xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans (2012), Lett. Appl. Microbiol., 54, 79-87.
    View publication on PubMed
Search for more literature for 3.2.1.37

Application

Application Comment Organism
biotechnology the bifunctional beta-glucosidase/xylosidase can be used in simultaneous saccharification of cellulose ans xylan into fermenantable glucose and xylose uncultured rumen bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 50°C, compared to KM-value: 0.164 mM for 4-nitrophenyl-beta-D-glucopyranoside of the beta-glucosidase activity, suggesting that RuBGX1 prefers 4-nitrophenyl-beta-D-xylopyranoside substrate uncultured rumen bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 purified RuBGX1-6His uncultured rumen bacterium

Organism

Organism UniProt Comment Textmining
uncultured rumen bacterium D3VYD8 RuBGX1, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21, three catalytic amino acids of RuBGX1 are identified , as D272 is the catalytic nucleophile, and E167 and E481 are the catalytic acids; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
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Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens uncultured rumen bacterium
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O 29% of its beta-glucosidase activity against p-nitrophenyl-beta-D-glucopyranoside uncultured rumen bacterium 4-nitrophenol + D-xylose
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 ?
additional information the hydrolysis of xylooligosaccharide substrates is investigated using an endoxylanase-coupled assay, functions synergistically to promote the degradation of hemicellulosic xylans uncultured rumen bacterium ?
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 ?

Synonyms

Synonyms Comment Organism
beta-xylosidase
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 uncultured rumen bacterium
bifunctional beta-glucosidase/xylosidase belongs to glycosyl hydrolase family 3, GH3 uncultured rumen bacterium
RuBGX1 gene name, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21 uncultured rumen bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at uncultured rumen bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at uncultured rumen bacterium