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Literature summary for 3.2.1.37 extracted from
- Expression and characterization of a thermostable beta-xylosidase from the hyperthermophile, Thermotoga maritima (2004), Biotechnol. Lett., 26, 1511-1515.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged enzyme in Escherichia coli | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.1, 90°C, recombinant enzyme | Thermotoga maritima |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 7fold from Escherichia coli by heat treatment and nickel affinity chromatography to homogeneity | Thermotoga maritima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal growth temperature is 80°C | Thermotoga maritima | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Thermotoga maritima |
| 50 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-alpha-L-arabinofuranoside | Thermotoga maritima |
| 275 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-xylopyranoside | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-xylose | - |
? | |
| xylan + H2O | from corncob, complete hydrolysis to beta-D-xylose in concert with xylanase | Thermotoga maritima | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylosidase | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal growth temperature is 80°C | Thermotoga maritima |
| 90 | - |
recombinant enzyme | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
1 h, pH 5.4-8.5, 57% activity remaining | Thermotoga maritima |
| 95 | - |
half-life 22 min, recombinant enzyme | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
recombinant enzyme | Thermotoga maritima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 8.5 | 1 h, 80°C, 57% activity remaining | Thermotoga maritima |
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