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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of an beta-D-xylosidase from Candida utilis IFO 0639 (2001), Biosci. Biotechnol. Biochem., 65, 527-533.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 10% v/v, 57% of stimulation | Cyberlindnera jadinii |  |
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | enzyme efficiently releases monoterpenols from an aroma precursor extracted from muscat grape juice | Cyberlindnera jadinii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | 1 mM, 3% residual activity | Cyberlindnera jadinii | |
| additional information | not inhibitory: xylose up to 0.3 M | Cyberlindnera jadinii | |
| p-chloromercuribenzoate | 1 mM, 2% residual activity | Cyberlindnera jadinii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Cyberlindnera jadinii | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
1 * 92000, SDS-PAGE | Cyberlindnera jadinii |
| 120000 | - |
gel filtration | Cyberlindnera jadinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
IFO 0639 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.4 | - |
30°C, pH 6.0 | Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 23% of activity compared to p-nitrophenyl-beta-D-xylopyranoside | Cyberlindnera jadinii | 4-nitrophenol + D-glucopyranose | - |
? | |
| additional information | no substrate: p-nitrophenyl-alpha-L-rhamnopyranoside, p-nitrophenyl-alpha-D-xylopyranoside | Cyberlindnera jadinii | ? | - |
? | |
| o-nitrophenyl-beta-D-xylopyranoside + H2O | 9% of activity compared to p-nitrophenyl-beta-D-xylopyranoside | Cyberlindnera jadinii | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | 5% of activity compared to p-nitrophenyl-beta-D-xylopyranoside | Cyberlindnera jadinii | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| p-nitrophenyl-beta-D-cellobioside + H2O | 6% of activity compared to p-nitrophenyl-beta-D-xylopyranoside | Cyberlindnera jadinii | p-nitrophenol + beta-D-cellobiose | - |
? | |
| p-nitrophenyl-beta-D-xylopyranoside + H2O | - |
Cyberlindnera jadinii | p-nitrophenol + beta-D-xylopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 92000, SDS-PAGE | Cyberlindnera jadinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Cyberlindnera jadinii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cyberlindnera jadinii | isoelectric focusing | - |
6 |
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