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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of extracellular beta-xylosidase and beta-glucosidase from Aspergillus fumigatus (1986), Agric. Biol. Chem., 50, 1703-1711.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong inhibition at 2 mM | Aspergillus fumigatus |  |
| Fe3+ | slight inhibition at 2 mM | Aspergillus fumigatus | |
| Hg2+ | complete inhibition at 2 mM | Aspergillus fumigatus | |
| p-chloromercuribenzoate | complete inhibition at 2 mM | Aspergillus fumigatus | |
| SDS | complete inhibition at 2 mM | Aspergillus fumigatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
p-nitrophenyl beta-D-xyloside | - |
Aspergillus fumigatus | |
| 23.8 | - |
xylobiose | - |
Aspergillus fumigatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus fumigatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
4 * 90000, SDS-PAGE | Aspergillus fumigatus |
| 360000 | - |
gel filtration, SDS-PAGE | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylo-oligosaccharides + H2O | Aspergillus fumigatus | - |
beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | Aspergillus fumigatus | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 4step chromatography | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Aspergillus fumigatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.2 | - |
- |
Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl beta-D-xyloside + H2O | - |
Aspergillus fumigatus | p-nitrophenol + beta-D-xylose | - |
? | |
| xylo-oligosaccharides + H2O | - |
Aspergillus fumigatus | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | - |
Aspergillus fumigatus | beta-D-xylose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 90000, SDS-PAGE | Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable up to | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus fumigatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | stable for 20 min at 50°C | Aspergillus fumigatus |
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