Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus pumilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-nitrophenyl beta-D-xyloside | inhibition at 6.2 mM | Bacillus pumilus | |
xylobiose | inhibition at 45 mM | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
p-nitrophenyl beta-D-xyloside | overexpressed enzyme | Bacillus pumilus | |
8.9 | - |
xylobiose | overexpressed enzyme | Bacillus pumilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Bacillus pumilus | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
gel filtration, SDS-PAGE | Bacillus pumilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylobiose + H2O | Bacillus pumilus | - |
beta-D-xylose + D-xylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by 6step chromatography techniques | Bacillus pumilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Bacillus pumilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.2 | - |
native enzyme | Bacillus pumilus |
28.3 | - |
overexpressed enzyme | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl beta-D-xyloside + H2O | - |
Bacillus pumilus | p-nitrophenol + beta-D-xylose | - |
? | |
xylobiose + H2O | - |
Bacillus pumilus | beta-D-xylose + D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | tetramer or trimer of 3-4 identical subunits of 56000-62000, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus pumilus |