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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of a thermostable beta-xylosidase from Thermoanaerobacter ethanolicus (1992), J. Bacteriol., 174, 5848-5853.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| o-nitrophenyl beta-D-xylopyranoside | substrate inhibition if concentration higher than 0.5 mM | Thermoanaerobacter ethanolicus |  |
| p-nitrophenyl alpha-L-arabinofuranoside | substrate inhibition if concentration higher than 12 mM | Thermoanaerobacter ethanolicus | |
| p-nitrophenyl alpha-L-arabinopyranoside | substrate inhibition if concentration higher than 9 mM | Thermoanaerobacter ethanolicus | |
| p-nitrophenyl beta-D-xylopyranoside | substrate inhibition if concentration higher than 0.1-0.2 mM | Thermoanaerobacter ethanolicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
p-nitrophenyl beta-D-xylopyranoside | at pH 5.0-5.2 | Thermoanaerobacter ethanolicus | |
| 0.038 | - |
p-nitrophenyl beta-D-xylopyranoside | at pH 5.8-6.0 | Thermoanaerobacter ethanolicus | |
| 0.06 | - |
o-nitrophenyl beta-D-xylopyranoside | - |
Thermoanaerobacter ethanolicus | |
| 2.1 | - |
p-nitrophenyl alpha-L-arabinopyranoside | - |
Thermoanaerobacter ethanolicus | |
| 4.6 | - |
p-nitrophenyl alpha-L-arabinofuranoside | - |
Thermoanaerobacter ethanolicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermoanaerobacter ethanolicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
2 * 85000, SDS-PAGE, native PAGE | Thermoanaerobacter ethanolicus |
| 165000 | - |
native PAGE, gel filtration, SDS-PAGE | Thermoanaerobacter ethanolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylobiose + H2O | Thermoanaerobacter ethanolicus | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter ethanolicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 3step chromatography techniques | Thermoanaerobacter ethanolicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | highest enzyme synthesis in sucrose/xylose medium | Thermoanaerobacter ethanolicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122 | - |
for p-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
| 183 | - |
for o-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
| 758 | - |
for p-nitrophenyl alpha-L-arabinopyranoside | Thermoanaerobacter ethanolicus |
| 1073 | - |
for p-nitrophenyl alpha-L-arabinofuranoside | Thermoanaerobacter ethanolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoanaerobacter ethanolicus | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| p-nitrophenyl alpha-L-arabinofuranoside + H2O | highest activity | Thermoanaerobacter ethanolicus | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| p-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Thermoanaerobacter ethanolicus | p-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoanaerobacter ethanolicus | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylobiose + H2O | - |
Thermoanaerobacter ethanolicus | beta-D-xylose + D-xylose | - |
? | |
| xylopentaose + H2O | lower activity than on xylobiose | Thermoanaerobacter ethanolicus | beta-D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 85000, SDS-PAGE, native PAGE | Thermoanaerobacter ethanolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside and p-nitrophenyl alpha-L-arabinopyranoside | Thermoanaerobacter ethanolicus |
| 82 | - |
at optimal pH 5.0-5.2 and hydrolysis of p-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
| 90 | - |
hydrolysis of o-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
| 93 | - |
at optimal pH 5.8-6.0 and hydrolysis of p-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
stable for more than 1 h, half-life at 86°C for 15 min | Thermoanaerobacter ethanolicus |
| 86 | - |
half-life 15 min | Thermoanaerobacter ethanolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.2 | at optimal temperature of 82°C and hydrolysis of p-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
| 5.8 | 6 | at optimal temperature of 93°C and hydrolysis of p-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
| 6 | - |
hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl beta-D-xylopyranoside | Thermoanaerobacter ethanolicus |
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