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Literature summary for 3.2.1.37 extracted from
- Biochemical properties of a beta-xylosidase from Clostridium cellulolyticum (1995), Appl. Environ. Microbiol., 61, 3509-3512.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
p-nitrophenyl beta-D-xyloside | - |
Ruminiclostridium cellulolyticum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Ruminiclostridium cellulolyticum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | Ruminiclostridium cellulolyticum | low activity | beta-D-xylose + ? | - |
? | |
| xylan + H2O | Ruminiclostridium cellulolyticum | complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | Ruminiclostridium cellulolyticum | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminiclostridium cellulolyticum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 3step chromatography techniques | Ruminiclostridium cellulolyticum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | enzyme production most induced in xylan medium | Ruminiclostridium cellulolyticum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.1 | - |
- |
Ruminiclostridium cellulolyticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl alpha-L-arabinofuranoside + H2O | low activity | Ruminiclostridium cellulolyticum | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Ruminiclostridium cellulolyticum | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylan + H2O | low activity | Ruminiclostridium cellulolyticum | beta-D-xylose + ? | - |
? | |
| xylan + H2O | complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose | Ruminiclostridium cellulolyticum | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | - |
Ruminiclostridium cellulolyticum | beta-D-xylose + D-xylose | - |
? | |
| xylopentaose + H2O | extend of hydrolysis varies with chain length of the substrate | Ruminiclostridium cellulolyticum | beta-D-xylose + ? | - |
? | |
| xylotetraose + H2O | acts by inverting the anomeric configuration | Ruminiclostridium cellulolyticum | xylotriose + beta-D-xylose | - |
? | |
| xylotriose + H2O | - |
Ruminiclostridium cellulolyticum | beta-D-xylose + xylobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Ruminiclostridium cellulolyticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Ruminiclostridium cellulolyticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Ruminiclostridium cellulolyticum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
no activity below | Ruminiclostridium cellulolyticum |
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