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Literature summary for 3.2.1.35 extracted from
- Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors (2007), Glycobiology, 17, 444-453.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)RIL cells and Drosophila melanogaster Schneider-2 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycyrrhizic acid | - |
Homo sapiens |  |
| L-Arginine-HCl | 8% residual activity at 1 mM | Homo sapiens | |
| L-ascorbic acid decanoate | - |
Homo sapiens | |
| L-ascorbic acid dodecanoate | - |
Homo sapiens | |
| L-ascorbic acid tridecanoate | - |
Homo sapiens | |
| L-ascorbic acid undecanoate | - |
Homo sapiens | |
| NaCl | complete inhibition at 1 mM | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48300 | - |
recombinant enzyme from Escherichia coli, SDS-PAGE | Homo sapiens |
| 54000 | - |
recombinant enzyme from Drosophila melanogaster Schneider-2 cells, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Ni-ion metal affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + 2 H2O | - |
Homo sapiens | N-acetyl-beta-D-glucosamine + D-glucuronate + ? | - |
? | |
| hyaluronic acid + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hyal-1 | - |
Homo sapiens |
| hyaluronidase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4 | - |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
at pH 3.5 | Homo sapiens | glycyrrhizic acid | |
| 0.05 | - |
at pH 3.5 | Homo sapiens | L-ascorbic acid tridecanoate | |
| 0.076 | - |
at pH 3.5 | Homo sapiens | L-ascorbic acid dodecanoate | |
| 0.143 | - |
at pH 3.5 | Homo sapiens | L-ascorbic acid undecanoate | |
| 0.378 | - |
at pH 3.5 | Homo sapiens | L-ascorbic acid decanoate | |
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