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Literature summary for 3.2.1.35 extracted from
- Isolation and characterization of hyaluronidase a "spreading factor" from Indian cobra (Naja naja) venom (2004), Biochimie, 86, 193-202.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | venom | Naja naja | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70410 | - |
mass spectrometry, isozyme NNH1 | Naja naja |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | Naja naja | absolutely specific for | tetrasaccharides units of hyaluronan | - |
? |  |
| additional information | Naja naja | the enzyme indirectly potentiates the myotoxicity of VRV-PL-VIII, a phospholipolytic myotoxin, and also the hemorrhagic potency of a hemorrhagic complex-I | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Naja naja | - |
two isozymes NNH1 and NNH2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozymes NNH1 and NNH2 33fold and 26fold, respectively, from venom by gel filtration and ion exchange chromatography | Naja naja |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Naja naja | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified isozymes NNH1 and NNH2 | Naja naja |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | absolutely specific for | Naja naja | tetrasaccharides units of hyaluronan | - |
? | |
| hyaluronan + H2O | absolutely specific for, cleavage of beta-1,4-glycosidic linkages, of human skin, extracellular matrix | Naja naja | tetrasaccharides units of hyaluronan | - |
? | |
| additional information | the enzyme indirectly potentiates the myotoxicity of VRV-PL-VIII, a phospholipolytic myotoxin, and also the hemorrhagic potency of a hemorrhagic complex-I | Naja naja | ? | - |
? | |
| additional information | no activity with chondroitin sulfates, heparin, chitosan, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, or 4-nitrophenyl-beta-D-glucuronide | Naja naja | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyaluronidase | - |
Naja naja |
| NNH1 | - |
Naja naja |
| NNH2 | - |
Naja naja |
| spreading factor | - |
Naja naja |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Naja naja |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Naja naja |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Naja naja | isoelectric focusing | - |
9.2 |
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Release 2023.1 (January 2023)
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