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Literature summary for 3.2.1.35 extracted from
- Hyaluronidase in sera of tumour-bearing nude mice (2003), Biomarkers, 8, 333-338.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | cancer cell lines often secrete hyaluronidase, the enzyme might therefore be a marker for growing tumours | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | enzyme activity in serum from tumour-bearing mice, e.g. H460M or SA87 cell grafted mice, is increased, the level is corrsponding to the tumour mass, not in serum from CB193 cell grafted mice | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
nude mice, 2 enzyme forms with one or five isoforms, respectively | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | from mice grafted with human-tumour-derived, enzyme secreting H460M or SA87 cells, and mice grafted with enzyme non-secreting CB 193 cells | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme activity in different serum samples | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | complete degradation, absolutely specific for | Mus musculus | N-acetylglucosamine + D-glucuronate + ? | - |
? | |
| additional information | enzyme activity in serum from tumour-bearing mice, e.g. H460M or SA87 cell grafted mice, is increased, the level is corrsponding to the tumour mass, not in serum from CB193 cell grafted mice | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyaluronidase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
inactivation | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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