Literature summary for 3.2.1.33 extracted from

Bates, E.J.; Heaton, G.M.; Taylor, C.; Kernohan, J.C.; Cohen, P.
Debranching enzyme from rabbit skeletal muscle: evidence for the location of two active centres on a single polypeptide chain (1975), FEBS Lett., 58, 181-185.

Search for more literature for 3.2.1.33

General Stability

General Stability	Organism
glucosidase: rather resistant to proteolytic attack: very little inactivation after prolonged incubation with 1% trypsin or 1% chymotrypsin	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
164000	-	glycogen debranching enzyme, sedimentation equilibrium	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glycogen debranching enzyme	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	skeletal	Oryctolagus cuniculus	-

Storage Stability

Storage Stability	Organism
0°C, 50 mM glycerophosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0, 2 months, without loss of activity	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycogen + H2O	reverse reaction: incorporation of glucose into glycogen	Oryctolagus cuniculus	glycogen + D-glucose	-	r
glycogen + H2O	slight reversibility	Oryctolagus cuniculus	glycogen + D-glucose	-	r
glycogen phosphorylase-limit dextrin + H2O	-	Oryctolagus cuniculus	limit dextrin + D-glucose	-	r

Subunits

Subunits	Comment	Organism
monomer	-	Oryctolagus cuniculus

Synonyms

Synonyms	Comment	Organism
glycogen debranching system	EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system	Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	completely stable for 20 h at 25°C in 50 mM glycerophosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0	Oryctolagus cuniculus

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Release 2023.1 (January 2023)