Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.33 extracted from

  • Taylor, C.; Cox, A.J.; Kernohan, J.C.; Cohen, P.
    Debranching enzyme from rabbit skeletal muscle. Purification, properties and physiological role (1975), Eur. J. Biochem., 51, 105-115.
    View publication on PubMed
Search for more literature for 3.2.1.33

Activating Compound

Activating Compound Comment Organism Structure
additional information no activating substance Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
glycogen particle protein-glycogen particle from skeletal muscle, glycogen debranching enzyme component of Oryctolagus cuniculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
 1 * 155000, glycogen debranching enzyme, sedimentation equilibrium with 6 M guanidinium chloride Oryctolagus cuniculus
164000
-
 glycogen debranching enzyme, sedimentation equilibrium Oryctolagus cuniculus
166000
-
 1 * 166000, glycogen debranching enzyme, SDS-PAGE Oryctolagus cuniculus
250000
-
 glycogen debranching enzyme, low-speed sedimentation equilibrium centrifugation, enzyme has tendency to associate from monomeric form, about 160000 Da, to higher molecular weight species at a protein concentration greater than about 5 mg/ml Oryctolagus cuniculus
267000 279000 enzyme has tendency to associate from monomeric form, about 160000 Da, to higher molecular weight species at a protein concentration greater than about 5 mg/ml Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glycogen debranching enzyme Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle skeletal Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
4°C, 50 mM glycerol-1-phosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0, 1 month, little or no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + H2O reverse reaction: incorporation of glucose into glycogen Oryctolagus cuniculus glycogen + D-glucose
-
 r
glycogen + H2O slight reversibility Oryctolagus cuniculus glycogen + D-glucose
-
 r
glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 155000, glycogen debranching enzyme, sedimentation equilibrium with 6 M guanidinium chloride Oryctolagus cuniculus
monomer 1 * 166000, glycogen debranching enzyme, SDS-PAGE Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus