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Literature summary for 3.2.1.33 extracted from

  • Brown, D.H.; Illingworth Brown, B.
    Enzymes of glycogen debranching: amylo-1,6-glucosidase (I) and oligo-1,4 -> 1,4-glucantransferase (II) (1966), Methods Enzymol., 8, 515-524.
No PubMed abstract available
Search for more literature for 3.2.1.33

Activating Compound

Activating Compound Comment Organism Structure
additional information no activating substance Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
glycylglycine slight inhibition Oryctolagus cuniculus
p-hydroxymercuribenzoate 0.3 mM: completely Oryctolagus cuniculus
Tris 0.02 M Tris: 50-60% inhibition Oryctolagus cuniculus
Urea glucosidase-transferase: 31% inhibition at 0.21 M, 72% at 1.6 M, 98% at 3.2 M Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Oryctolagus cuniculus
0.74
-
 glycogen phosphorylase limit dextrin
-
 Oryctolagus cuniculus
1.8
-
 63-alpha-glucosyl maltotetraose branched pentasaccharide B5, pH 6.6 Oryctolagus cuniculus
5.6
-
 63-alpha-maltotriosyl maltotetraose branched heptasaccharide B7, muscle enzyme Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
267000 279000 glycogen debranching enzyme, equilibrium ultracentrifugation Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus
0.82
-
 substrate: 63-alpha-glucosyl maltotetraose Oryctolagus cuniculus
3.9
-
 substrate: limit dextrin Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
63-alpha-maltotriosyl maltotetraose + H2O branched heptasaccharide B7 Oryctolagus cuniculus ?
-
 ?
beta-amylase limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
glycogen phosphorylase limit dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose partially debranched glycogen r
additional information
-
 Oryctolagus cuniculus ?
-
 ?

Synonyms

Synonyms Comment Organism
glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1 6.4 sodium citrate/mercaptoethanol buffer Oryctolagus cuniculus