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Literature summary for 3.2.1.33 extracted from
- Purification and properties of yeast amylo-1,6-glucosidase-oligo-1,4 -> 1,4-glucantransferase (1970), Biochemistry, 9, 2347-2355.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 210000 | - |
glycogen debranching enzyme, gel filtration and PAGE | Saccharomyces cerevisiae |
| 280000 | - |
glycogen debranching enzyme, sedimentation equilibrium | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen phosphorylase-limit dextrin + H2O | Saccharomyces cerevisiae | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glycogen debranching enzyme | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Saccharomyces cerevisiae |
| 8.4 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-(1-6)-glucosyl cycloheptaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Saccharomyces cerevisiae | cycloheptaamylose + D-glucose | alpha-Schardinger dextrin | r | |
| alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Saccharomyces cerevisiae | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
| amylopectin + H2O | slowly hydrolyzed | Saccharomyces cerevisiae | amylopectin + D-glucose | - |
r | |
| D-glucose + maltooligosaccharide | maltotetraose and maltopentaose | Saccharomyces cerevisiae | maltooligosaccharide | branched | r | |
| glycogen + H2O | native glycogen from shellfish and rabbit liver | Saccharomyces cerevisiae | glycogen + D-glucose | - |
r | |
| glycogen phosphorylase-limit dextrin + H2O | - |
Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
| glycogen phosphorylase-limit dextrin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.6 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 25% of maximum activity, pH 8.0: about 40% of maximum activity | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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