General Stability | Organism |
---|---|
glucosidase-transferase stable in 0.05 M Tris and 2 M urea, up to 30°C | Oryctolagus cuniculus |
glucosidase-transferase stable in 2 M urea, 2 M urea for prolonged periods of time: no loss in activity | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glycogen debranching enzyme | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.78 | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Oryctolagus cuniculus | maltotetraose + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | - |
Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
up to, in the presence of Tris and urea | Oryctolagus cuniculus |
45 | - |
complete loss of activity | Oryctolagus cuniculus |