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Literature summary for 3.2.1.33 extracted from

  • Lee, E.Y.C.; Carter, J.H.
    Amylo-1,6-glucosidase/1,4-alpha-glucan: 1,4-alpha-glucan 4-alpha-glycosyltransferase: specificity toward polysaccharide substrates (1973), Arch. Biochem. Biophys., 154, 636-641.
    View publication on PubMed
Search for more literature for 3.2.1.33

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
 glycogen phosphorylase limit dextrin phi-dextrin from shellfish glycogen Oryctolagus cuniculus
1.6
-
 glycogen phosphorylase limit dextrin phi-dextrin from shellfish glycogen Saccharomyces cerevisiae
3.8
-
 beta-amylase limit dextrin beta-dextrin from shellfish glycogen Saccharomyces cerevisiae
4.3
-
 amylopectin beta-dextrin
-
 Saccharomyces cerevisiae
7.2
-
 beta-amylase limit dextrin beta-dextrin from shellfish glycogen Oryctolagus cuniculus
11
-
 amylopectin beta-dextrin
-
 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycogen phosphorylase-limit dextrin + H2O Saccharomyces cerevisiae phi-dextrin ?
-
 ?
glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus phi-dextrin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glycogen debranching enzyme Saccharomyces cerevisiae
glycogen debranching enzyme Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
-
 Saccharomyces cerevisiae
7.6
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O very poor substrate Oryctolagus cuniculus amylopectin + D-glucose partially debranched r
amylopectin + H2O phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin Saccharomyces cerevisiae amylopectin + D-glucose partially debranched r
amylopectin + H2O phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin Oryctolagus cuniculus amylopectin + D-glucose partially debranched r
amylopectin beta-dextrin + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
amylopectin beta-dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
amylose + H2O
-
 Saccharomyces cerevisiae ?
-
 r
amylose + H2O
-
 Oryctolagus cuniculus ?
-
 r
beta-amylase limit dextrin + H2O beta-dextrin Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
beta-amylase limit dextrin + H2O beta-dextrin Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
glycogen + H2O rabbit liver glycogen: very poor substrate, shellfish glycogen: not a substrate Oryctolagus cuniculus glycogen + D-glucose
-
 r
glycogen + H2O reverse reaction: synthesis of (1, 6)-bound side chains, reincorporation of glucose into polysaccharide Saccharomyces cerevisiae glycogen + D-glucose
-
 r
glycogen + H2O reverse reaction: synthesis of (1, 6)-bound side chains, reincorporation of glucose into polysaccharide Oryctolagus cuniculus glycogen + D-glucose
-
 r
glycogen + H2O native glycogen from shellfish and rabbit liver Saccharomyces cerevisiae glycogen + D-glucose
-
 r
glycogen + H2O reverse reaction: incorporation of glucose into glycogen Saccharomyces cerevisiae glycogen + D-glucose
-
 r
glycogen + H2O reverse reaction: incorporation of glucose into glycogen Oryctolagus cuniculus glycogen + D-glucose
-
 r
glycogen phosphorylase limit dextrin + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
glycogen phosphorylase limit dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
glycogen phosphorylase-limit dextrin + H2O phi-dextrin Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
glycogen phosphorylase-limit dextrin + H2O phi-dextrin Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
glycogen phosphorylase-limit dextrin + H2O phi-dextrin Saccharomyces cerevisiae ?
-
 ?
glycogen phosphorylase-limit dextrin + H2O phi-dextrin Oryctolagus cuniculus ?
-
 ?
additional information substrate specificities Saccharomyces cerevisiae ?
-
 ?
additional information substrate specificities Oryctolagus cuniculus ?
-
 ?
phosphorylase beta-amylase limit dextrin + H2O limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
phosphorylase beta-amylase limit dextrin + H2O limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin Oryctolagus cuniculus limit dextrin + D-glucose
-
 r

Synonyms

Synonyms Comment Organism
glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Saccharomyces cerevisiae
glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus