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Literature summary for 3.2.1.32 extracted from

  • Cai, Z.W.; Ge, H.H.; Yi, Z.W.; Zeng, R.Y.; Zhang, G.Y.
    Characterization of a novel psychrophilic and halophilic beta-1,3-xylanase from deep-sea bacterium, Flammeovirga pacifica strain WPAGA1 (2018), Int. J. Biol. Macromol., 118, 2176-2184 .
    View publication on PubMed
Search for more literature for 3.2.1.32

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10 mM, activation to 105.32% of control Flammeovirga pacifica

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Flammeovirga pacifica

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 10 mM, 32% loss of activity Flammeovirga pacifica
Cu2+ 10 mM, 63% loss of activity Flammeovirga pacifica
Fe3+ 10 mM, 64% loss of activity Flammeovirga pacifica
K+ 10 mM, 3% loss of activity Flammeovirga pacifica
Mn2+ 10 mM, 7% loss of activity Flammeovirga pacifica
Ni2+ 10 mM, 59% loss of activity Flammeovirga pacifica
Zn2+ 10 mM, 66% loss of activity Flammeovirga pacifica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.32
-
 glycol beta-1,3-xylan 20°C, pH 7.5, 1.5 mM NaCl Flammeovirga pacifica
5.06
-
 glycol beta-1,3-xylan 20°C, pH 7.5, 0 mM NaCl Flammeovirga pacifica
5.41
-
 beta-1,3-xylan 20°C, pH 7.5, 0 mM NaCl Flammeovirga pacifica
6.01
-
 beta-1,3-xylan 20°C, pH 7.5, 1.5 mM NaCl Flammeovirga pacifica

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, activation to 104.65% of control Flammeovirga pacifica
Ca2+ 10 mM, activation to 113.13% of control Flammeovirga pacifica
Mg2+ 10 mM, activation to 118.73% of control Flammeovirga pacifica
Na+ 10 mM, activation to 121.5% of control Flammeovirga pacifica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40510
-
 calculated from sequence Flammeovirga pacifica

Organism

Organism UniProt Comment Textmining
Flammeovirga pacifica A0A1S1YTI6
-
-
Flammeovirga pacifica WPAGA1 A0A1S1YTI6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flammeovirga pacifica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-xylan + H2O
-
 Flammeovirga pacifica ?
-
 ?
beta-1,3-xylan + H2O
-
 Flammeovirga pacifica WPAGA1 ?
-
 ?
glycol beta-1,3-xylan + H2O
-
 Flammeovirga pacifica ?
-
 ?
glycol beta-1,3-xylan + H2O
-
 Flammeovirga pacifica WPAGA1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Xyl512
-
 Flammeovirga pacifica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 without NaCl Flammeovirga pacifica
30
-
 in the present of 1.5 mol/l NaCl Flammeovirga pacifica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the thermal stability of the Xyl512 improves significantly. The enzyme entirely loses its activity after incubating at 40°C for 60 min without NaCl, but it remains 80% of activity after being incubated for 90 min in the presence of 1.5 mol/l NaCl Flammeovirga pacifica
20
-
 the enzyme is stable at 20°C after incubating for 90 min at pH 7.0 Flammeovirga pacifica
30
-
 the enzyme loses more than 60% of its original activity at 30°C for 30 min Flammeovirga pacifica
40
-
 the enzyme loses more than 80% of its original activity at 40°C for 15 min Flammeovirga pacifica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
529.22
-
 beta-1,3-xylan 20°C, pH 7.5, 0 mM NaCl Flammeovirga pacifica
529.22
-
 glycol beta-1,3-xylan 20°C, pH 7.5, 0 mM NaCl Flammeovirga pacifica
837.11
-
 glycol beta-1,3-xylan 20°C, pH 7.5, 1.5 mM NaCl Flammeovirga pacifica
895.45
-
 beta-1,3-xylan 20°C, pH 7.5, 1.5 mM NaCl Flammeovirga pacifica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 without NaCl Flammeovirga pacifica
7.5
-
 in the present of 1.5 mol/l NaCl Flammeovirga pacifica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
97.82
-
 beta-1,3-xylan 20°C, pH 7.5, 0 mM NaCl Flammeovirga pacifica
104.59
-
 glycol beta-1,3-xylan 20°C, pH 7.5, 0 mM NaCl Flammeovirga pacifica
148.99
-
 beta-1,3-xylan 20°C, pH 7.5, 1.5 mM NaCl Flammeovirga pacifica
193.77
-
 glycol beta-1,3-xylan 20°C, pH 7.5, 1.5 mM NaCl Flammeovirga pacifica