Cloned (Comment) | Organism |
---|---|
gene xyl4, DNA and amino acid sequence determination and comparison, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Vibrio sp. AX-4 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of five different XYL4 deletion mutants, CBM-1, CBM-2, CBM-1-2, CM-CBM-1, and CM, possessing a catalytic module | Vibrio sp. AX-4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding specificities and dissociation constants of XYL4 and its mutants with different polysaccharides and glycol-beta-1,3-xylan and beta-1,3-xylan, overview | Vibrio sp. AX-4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | both the enzyme and the binding activities for insoluble beta-1,3-xylan but not soluble glycol-beta-1,3-xylan, are enhanced by NaCl | Vibrio sp. AX-4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-xylan + H2O | Vibrio sp. AX-4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. AX-4 | D5MP61 | gene xyl4 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL2(DE3) by nickel affinity and beta-1,3-xylan affinity chromatography and gel filtration | Vibrio sp. AX-4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-xylan + H2O | - |
Vibrio sp. AX-4 | ? | - |
? | |
additional information | XYL4, an endo-type enzyme, contains two putative carbohydrate-binding modules, CBMs, belonging to family 31 in the C-terminal region. Repeating CBMs bind specifically to insoluble beta-1,3-xylan, but not to beta-1,4-xylan, Avicel, beta-1,4-mannan, curdlan, chitin or soluble glycol-beta-1,3-xylan, through hydrophobic interaction. Binding specificities of XYL4 and its mutants, overview | Vibrio sp. AX-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | XYL4 is a modular enzyme composed of an N-terminal catalytic module belonging to glycoside hydrolase family 26 and two putative carbohydrate-binding modules belonging to family 31 in the C-terminal region, sequence determination, overview | Vibrio sp. AX-4 |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-xylanase | - |
Vibrio sp. AX-4 |
More | XYL4 is a modular enzyme composed of an N-terminal catalytic module belonging to glycoside hydrolase family 26 and two putative carbohydrate-binding modules belonging to family 31 in the C-terminal region | Vibrio sp. AX-4 |
XYL4 | - |
Vibrio sp. AX-4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vibrio sp. AX-4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Vibrio sp. AX-4 |