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Literature summary for 3.2.1.32 extracted from
- Enzymatic properties of a neutral endo-1,3(4)-beta-xylanase Xyl II from Bacillus subtilis (2002), J. Biotechnol., 94, 265-275.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
x * 20000, SDS-PAGE, x * 20000-24000, gel filtration | Bacillus subtilis |
| 340000 | - |
PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
CCMI 966, enzyme Xyl II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 207 | - |
xylan (oat spelts), pH 6.0, 30°C | Bacillus subtilis |
| 225 | - |
xylan (birchwood), pH 6.0, 30°C | Bacillus subtilis |
| 226 | - |
laminarin, pH 6.0, 30°C | Bacillus subtilis |
| 253 | - |
xylan (larchwood), pH 6.0, 30°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Bacillus subtilis | ? | - |
? |  |
| laminarin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| xylan (birchwood) + H2O | - |
Bacillus subtilis | xylotetraose + ? | xylotetraose is main product of xylan degradation | ? | |
| xylan (larchwood) + H2O | - |
Bacillus subtilis | xylotetraose + ? | xylotetraose is main product of xylan degradation | ? | |
| xylan (oat spelts) + H2O | - |
Bacillus subtilis | xylotetraose + ? | xylotetraose is main product of xylan degradation | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme shows no xylan-binding domain | Bacillus subtilis |
| multimer | x * 20000, SDS-PAGE, x * 20000-24000, gel filtration | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
activity increases with temperature, denaturation above | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
180 min, stable | Bacillus subtilis |
| 50 | - |
92 min, 50% loss of activity | Bacillus subtilis |
| 60 | - |
20 min, 50% loss of activity | Bacillus subtilis |
| 70 | - |
8 min, 50% loss of activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoelectric focusing | - |
7.5 |
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