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Literature summary for 3.2.1.3 extracted from
- Glucoamylase from the predacious fungus Arthrobotrys conoides a cationic enzyme with high debranching activity and raw starch digestibility (2016), Appl. Biochem. Microbiol., 52, 176-182 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acarbose | 84% inhibition at 0.004 mg/ml, 66% at 0.002 mg/ml | Arthrobotrys conoides |  |
| Cu2+ | - |
Arthrobotrys conoides | |
| diethyl dicarbonate | 48% inhibition at 4 mM, 76% at 10 mM | Arthrobotrys conoides | |
| EDTA | 51% inhibition at 2 mM | Arthrobotrys conoides | |
| Hg2+ | 46% inhibition at 0.002 mM | Arthrobotrys conoides | |
| N-bromosuccinimide | 78% inhibition at 0.01 mM | Arthrobotrys conoides | |
| Pb2+ | - |
Arthrobotrys conoides | |
| Periodate | 27% inhibition at 5 mM, 30% at 10 mM, 35% at 15 mM. 34% Glycosyl content of the enzyme is lost at 2.1 mM of periodate | Arthrobotrys conoides | |
| Rose bengal | 48% inhibition at 0.25 mg/ml | Arthrobotrys conoides | |
| Zn2+ | - |
Arthrobotrys conoides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM values for amylopectin, glycogen and starch are 0.056, 0.062 and 0.065 mg/ml, respectively | Arthrobotrys conoides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Arthrobotrys conoides | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 23% activation at 4 mM | Arthrobotrys conoides | |
| additional information | Mg2+ and Mn2+ have negligible effect on the enzyme activity | Arthrobotrys conoides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
native enzyme, gel filtration | Arthrobotrys conoides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Arthrobotrys conoides | - |
D-glucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobotrys conoides | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content of the enzyme is 26.3%, 34% glycosyl content is lost at 2.1 mM of periodate | Arthrobotrys conoides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 68.2% by dialysis, gel filtration and anion exchange chromatography, more than 90% of native GA A binds to raw starch | Arthrobotrys conoides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 165.8 | - |
purified native enzyme, pH 5.6, 50°C | Arthrobotrys conoides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | best substrate | Arthrobotrys conoides | D-glucose + ? | - |
? | |
| additional information | the enzyme shows high affinity for the branched polysaccharides. It can digest raw starch | Arthrobotrys conoides | ? | - |
? | |
| starch + H2O | - |
Arthrobotrys conoides | D-glucose + ? | - |
? | |
| starch + H2O | soluble and raw starch | Arthrobotrys conoides | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 83500, native enzyme, SDS-PAGE | Arthrobotrys conoides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GA A | - |
Arthrobotrys conoides |
| glycoamylase | - |
Arthrobotrys conoides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Arthrobotrys conoides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified native enzyme, 30 min, stable | Arthrobotrys conoides |
| 45 | - |
purified native enzyme, 60 min, 59% activity remaining | Arthrobotrys conoides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 5.8 | - |
Arthrobotrys conoides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range, profile overview | Arthrobotrys conoides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 9 | purified native enzyme, stable at | Arthrobotrys conoides |
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