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Literature summary for 3.2.1.3 extracted from
- Glucoamylases from Penicillium verruculosum and Myceliophthora thermophila analysis of differences in activity against polymeric substrates based on 3D model structures of the intact enzymes (2015), Biochimie, 110, 45-51 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Penicillium canescens | Talaromyces verruculosus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| modeling of structure based on PDB entry 2vn7 | Talaromyces verruculosus |
| modeling of structure based on PDB entry 2vn7 | Thermothelomyces thermophilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Talaromyces verruculosus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces verruculosus | A0A1L5IUR2 | - |
- |
| Thermothelomyces thermophilus | G2QPS0 | - |
- |
| Thermothelomyces thermophilus DSM 1799 | G2QPS0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermothelomyces thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
substrate maltose, pH 4.7, 40°C | Talaromyces verruculosus |
| 3.6 | - |
substrate amylose, pH 4.7, 58°C | Talaromyces verruculosus |
| 4.7 | - |
substrate starch, pH 4.7, 30°C | Talaromyces verruculosus |
| 4.8 | - |
substrate amylopectin, pH 4.7, 58°C | Talaromyces verruculosus |
| 5.2 | - |
substrate maltose, pH 4.7, 40°C | Thermothelomyces thermophilus |
| 7.8 | - |
substrate amylose, pH 4.7, 58°C | Thermothelomyces thermophilus |
| 9.5 | - |
substrate amylopectin, pH 4.7, 58°C | Thermothelomyces thermophilus |
| 25 | - |
substrate starch, pH 4.7, 30°C | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Talaromyces verruculosus | ? | - |
? |  |
| amylopectin + H2O | - |
Thermothelomyces thermophilus | ? | - |
? | |
| amylopectin + H2O | - |
Thermothelomyces thermophilus DSM 1799 | ? | - |
? | |
| amylose + H2O | - |
Talaromyces verruculosus | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Thermothelomyces thermophilus | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Thermothelomyces thermophilus DSM 1799 | D-glucose + ? | - |
? | |
| maltose + H2O | - |
Talaromyces verruculosus | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Thermothelomyces thermophilus DSM 1799 | 2 D-glucose | - |
? | |
| additional information | no substrate: 4-nitrophenyl alpha-D-glucopyranoside | Talaromyces verruculosus | ? | - |
? | |
| additional information | no substrate: 4-nitrophenyl alpha-D-glucopyranoside | Thermothelomyces thermophilus | ? | - |
? | |
| additional information | no substrate: 4-nitrophenyl alpha-D-glucopyranoside | Thermothelomyces thermophilus DSM 1799 | ? | - |
? | |
| starch + H2O | - |
Talaromyces verruculosus | D-glucose + ? | - |
? | |
| starch + H2O | - |
Thermothelomyces thermophilus | D-glucose + ? | - |
? | |
| starch + H2O | - |
Thermothelomyces thermophilus DSM 1799 | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, SDS-PAGE, x * 65989, calculated | Talaromyces verruculosus |
| ? | x * 88000, SDS-PAGE, x * 65989, calculated | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gla1 | - |
Talaromyces verruculosus |
| Gla1 | - |
Thermothelomyces thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Talaromyces verruculosus |
| 5.5 | - |
- |
Thermothelomyces thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
90% of maximum activity | Talaromyces verruculosus |
| 4.7 | - |
90% of maximum activity | Thermothelomyces thermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Talaromyces verruculosus | isoelectric focusing | - |
3.8 |
| Thermothelomyces thermophilus | calculated | - |
3.8 |
| Talaromyces verruculosus | calculated | - |
4.6 |
| Thermothelomyces thermophilus | isoelectric focusing | - |
5.1 |
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