Literature summary for 3.2.1.3 extracted from

Cereia, M.; Guimaraes, L.; Peixoto-Nogueira, S.; Jorge, J.; Terenzi, H.; Greene, L.; Polizeli, M.
Glucoamylase isoform (GAII) purified from a thermophilic fungus Scytalidium thermophilum 15.8 with biotechnological potential (2006), Afr. J. Biotechnol., 5, 1239-1245 .

No PubMed abstract available

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Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	1 mM, complete loss of activity	Mycothermus thermophilus
Cu2+	10 mM, 71% residual activity with substrate starch, 31% with substrate maltose	Mycothermus thermophilus
Hg2+	10 mM, 10% residual activity	Mycothermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	starch	Km value 0.2 mg/ml, pH 5.5, 60°C	Mycothermus thermophilus
additional information	-	maltose	Km value 1.5 mg/ml, pH 5.5, 60°C	Mycothermus thermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Mycothermus thermophilus	-	-

Organism

Organism	UniProt	Comment	Textmining
Mycothermus thermophilus	-	-	-
Mycothermus thermophilus 15.8	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	10% carbohydrate content	Mycothermus thermophilus

Purification (Commentary)

Purification (Comment)	Organism
-	Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose + H2O	-	Mycothermus thermophilus	2 D-glucose	-	?
maltose + H2O	-	Mycothermus thermophilus 15.8	2 D-glucose	-	?
starch + H2O	-	Mycothermus thermophilus	D-glucose + ?	-	?
starch + H2O	-	Mycothermus thermophilus 15.8	D-glucose + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 83000,SDS-PAGE	Mycothermus thermophilus

Synonyms

Synonyms	Comment	Organism
GAII	-	Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Mycothermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Mycothermus thermophilus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Mycothermus thermophilus	isoelectric focusing	-	7.2

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Release 2023.1 (January 2023)