Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.3 extracted from

  • Li, Z.; Wei, P.; Cheng, H.; He, P.; Wang, Q.; Jiang, N.
    Functional role of beta domain in the Thermoanaerobacter tengcongensis glucoamylase (2014), Appl. Microbiol. Biotechnol., 98, 2091-2099.
    View publication on PubMed
Search for more literature for 3.2.1.3

Protein Variants

Protein Variants Comment Organism
I339G about 10% of wild-type specific activity Caldanaerobacter subterraneus subsp. tengcongensis
additional information presence of the beta domain is essential for catalytic activity of the enzyme. The catalytic domain alone is not able to hydrolyze soluble starch while starch hydrolysis activity is restored in the catalytic domain in the presence of the beta domain. The catalytic domain displays lower thermostability compared with the intact wild-type and exhibits enhanced thermostability in the presence of the beta domain in vitro. Truncation of the wild-type enzyme or mutagenesis of the residues that participate in the interdomain interaction at its beta domain also lead to the reduced thermostability of the enzyme Caldanaerobacter subterraneus subsp. tengcongensis
T47A about 50% of wild-type specific activity Caldanaerobacter subterraneus subsp. tengcongensis
T47A/W48A about 25% of wild-type specific activity Caldanaerobacter subterraneus subsp. tengcongensis
W48A about 55% of wild-type specific activity Caldanaerobacter subterraneus subsp. tengcongensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 starch Km value of wild-type is 34.5 mg/ml, pH 4.6, 70°C, of mutant T47A 61.1 mg/ml, mutant W48A 52.9 mg/ml Caldanaerobacter subterraneus subsp. tengcongensis

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis Q8R917
-
-
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 Q8R917
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
 mutant I339A, pH 4.6, 70°C Caldanaerobacter subterraneus subsp. tengcongensis
9
-
 mutant T47A/W48A, pH 4.6, 70°C Caldanaerobacter subterraneus subsp. tengcongensis
18.4
-
 mutant T47A, pH 4.6, 70°C Caldanaerobacter subterraneus subsp. tengcongensis
19.9
-
 mutant W48A, pH 4.6, 70°C Caldanaerobacter subterraneus subsp. tengcongensis
36.6
-
 wild-type, pH 4.6, 70°C Caldanaerobacter subterraneus subsp. tengcongensis
37.1
-
 beta domain plus catalytic domain independently expressed, pH 4.6, 70°C Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis beta-D-glucose + ?
-
 ?
starch + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 beta-D-glucose + ?
-
 ?