KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics and kinetics of starch hydrolysis, overview | Aspergillus niger | |
0.16 | - |
starch | mutant enzyme, pH 5.0, 40°C | Aspergillus niger | |
0.25 | - |
starch | wild-type enzyme, pH 5.0, 40°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72063 | - |
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry | Aspergillus niger |
72876 | - |
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry | Aspergillus niger |
93000 | - |
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
locally isolated Aspergillus niger, wild-type and deoxy-D-glucose-resistant mutant | - |
Purification (Comment) | Organism |
---|---|
glucoamylases from a wild-type and a deoxy-D-glucose-resistant mutant Aspergillus niger to apparent homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography, the wild-tyype enzyme is purified 25.4fold, the mutant 30.6fold | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
216 | - |
purified wild-type enzyme, pH 5.0, 40°C, substrate starch | Aspergillus niger |
495.2 | - |
purified mutant enzyme, pH 5.0, 40°C, substrate starch | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Aspergillus niger | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | activity range | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
343 | - |
starch | wild-type enzyme, pH 5.0, 40°C | Aspergillus niger | |
727 | - |
starch | mutant enzyme, pH 5.0, 40°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | activity range | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1347 | - |
starch | wild-type enzyme, pH 5.0, 40°C | Aspergillus niger | |
4510 | - |
starch | mutant enzyme, pH 5.0, 40°C | Aspergillus niger |