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Literature summary for 3.2.1.3 extracted from

  • Koc, O.; Metin, K.
    Purification and characterization of a thermostable glucoamylase produced by Aspergillus flavus HBF34 (2010), Afr. J. Biotechnol., 9, 3414-3424.
No PubMed abstract available
Search for more literature for 3.2.1.3

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
 Aspergillus flavus
Cu2+
-
 Aspergillus flavus
EDTA
-
 Aspergillus flavus
Fe3+
-
 Aspergillus flavus
Hg2+
-
 Aspergillus flavus
Mg2+
-
 Aspergillus flavus
N-bromosuccinimide
-
 Aspergillus flavus
Na2O3Se
-
 Aspergillus flavus
NH4Cl
-
 Aspergillus flavus
Ni2+
-
 Aspergillus flavus
Phenylmethanesulfonylfluoride
-
 Aspergillus flavus
Zn2+
-
 Aspergillus flavus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Aspergillus flavus
Ca2+ activates Aspergillus flavus
Co2+ activates at 1 mM, no effect at 10 mM Aspergillus flavus
Mn2+ activates Aspergillus flavus
additional information no effects on enzyme activity by Li+ and K+, and by NaCl Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
 two isozymes
-
Aspergillus flavus HBF34
-
 two isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes 120fold by starch affinity chromatography, isozyme separation by PAGE zymography Aspergillus flavus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus flavus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6619
-
 purified enzyme, pH 6.0, 60°C Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is active on the following substrates in descending order: glycogen, amylopectin, corn starch, rice starch, wheat starch, maltose, amylose, dextrin, maltotriose, raffinose, and sucrose Aspergillus flavus ?
-
 ?
additional information the enzyme is active on the following substrates in descending order: glycogen, amylopectin, corn starch, rice starch, wheat starch, maltose, amylose, dextrin, maltotriose, raffinose, and sucrose Aspergillus flavus HBF34 ?
-
 ?
soluble potato starch + H2O
-
 Aspergillus flavus ?
-
 ?
soluble potato starch + H2O
-
 Aspergillus flavus HBF34 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus flavus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity increases linearly from 10°C to 60°C and reaches a maximum at 60°C Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
27
-
 purified isozymes, 72 h, no loss in activity Aspergillus flavus
40
-
 purified isozymes, 72 h, no loss in activity Aspergillus flavus
50
-
 purified isozymes, 72 h, 46% activity remaining Aspergillus flavus
65
-
 purified isozymes, 95% activity remaining Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus flavus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 purified isozymes, 72 h, 78% activity remaining Aspergillus flavus

General Information

General Information Comment Organism
physiological function glucoamylase is an exo-acting enzyme that catalyses the production of beta-glucose from the non-reducing ends of amylose, amylopectin and glycogen Aspergillus flavus