Literature summary for 3.2.1.3 extracted from

da Silva, T.M.; Maller, A.; Damasio, A.R.; Michelin, M.; Ward, R.J.; Hirata, I.Y.; Jorge, J.A.; Terenzi, H.F.; de Polizeli, M.L.
Properties of a purified thermostable glucoamylase from Aspergillus niveus (2009), J. Ind. Microbiol. Biotechnol., 36, 1439-1446.

Search for more literature for 3.2.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
beta-mercaptoethanol	1 mM	Aspergillus niveus
EDTA	1 mM	Aspergillus niveus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	21% inhibition of enzyme activity	Aspergillus niveus
Fe2+	5% inhibition of enzyme activity	Aspergillus niveus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	starch	0.32 mg/ml	Aspergillus niveus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
BaCl2	1 mM	Aspergillus niveus
CaCl2	1 mM	Aspergillus niveus
CoCl2	1 mM	Aspergillus niveus
CuCl2	1 mM	Aspergillus niveus
KCl	1 mM	Aspergillus niveus
KH2PO4	1 mM	Aspergillus niveus
MgCl2	1 mM	Aspergillus niveus
MnCl2	1 mM	Aspergillus niveus
NaBr	1 mM	Aspergillus niveus
NaCl	1 mM	Aspergillus niveus
NaH2PO4	1 mM	Aspergillus niveus
NH4Cl	1 mM	Aspergillus niveus
NH4F	1 mM	Aspergillus niveus
ZnCl2	1 mM	Aspergillus niveus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
76000	-	Bio-Sil-Sec-400 gel filtration	Aspergillus niveus
77000	-	SDS-PAGE	Aspergillus niveus

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	10%, enzyme remains competely active	Aspergillus niveus
Ethanol	10%, enzyme maintains high activtiy	Aspergillus niveus
isopropanol	10%, enzyme maintains high activity	Aspergillus niveus
Methanol	10%, enzyme remains completely active	Aspergillus niveus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niveus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
2.53fold by DEAE-Fractogel and Concanacalin A-Sepharose chromatography	Aspergillus niveus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
600	-	after purification	Aspergillus niveus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
starch + H2O	-	Aspergillus niveus	D-glucose + ?	-	?

Synonyms

Synonyms	Comment	Organism
1,4-alpha-D-glucan-glucohydrolase	-	Aspergillus niveus
glucoamylase	-	Aspergillus niveus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Aspergillus niveus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	100% enzyme activity after 240 min at 60°C	Aspergillus niveus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
14.2	-	starch	-	Aspergillus niveus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	5.5	-	Aspergillus niveus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	9.5	enzyme remains stable for at least 2 h	Aspergillus niveus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Aspergillus niveus	-	-	3.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	starch	44.4 ml/sec*mg	Aspergillus niveus

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Release 2023.1 (January 2023)