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Literature summary for 3.2.1.3 extracted from

  • Zheng, Y.; Xue, Y.; Zhang, Y.; Zhou, C.; Schwaneberg, U.; Ma, Y.
    Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4 (2010), Appl. Microbiol. Biotechnol., 87, 225-233.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caldanaerobacter subterraneus subsp. tengcongensis MB4

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
EDTA
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
Pb2+
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
Zn2+
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.35
-
maltotetraose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
1.63
-
maltopentaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
1.88
-
maltohexaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
1.93
-
maltotriose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
2.21
-
maltoheptaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
2.41
-
isomaltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
13.4
-
maltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight increase in enzyme activtiy Caldanaerobacter subterraneus subsp. tengcongensis MB4
Mn2+ slight increase in enzyme activity Caldanaerobacter subterraneus subsp. tengcongensis MB4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis MB4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purification to 30fold homogeneity by heat treatment and gel filtration chromatography Caldanaerobacter subterraneus subsp. tengcongensis MB4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
175
-
after purification Caldanaerobacter subterraneus subsp. tengcongensis MB4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
maltoheptaose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
maltohexaose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
maltopentaose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
maltose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
maltotetraose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
maltotriose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Synonyms

Synonyms Comment Organism
glucoamylase
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
TtcGA
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
no activity loss after incubation at 75°C for 6 h, half life at 80°C 2.6 h Caldanaerobacter subterraneus subsp. tengcongensis MB4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.7
-
isomaltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
149
-
maltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
343
-
maltoheptaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
368
-
maltotriose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
459
-
maltohexaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
460
-
maltotetraose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
518
-
maltopentaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 above and below abruptly loss of enzyme activity Caldanaerobacter subterraneus subsp. tengcongensis MB4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.44
-
isomaltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
11.1
-
maltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
155
-
maltoheptaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
191
-
maltotriose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
244
-
maltohexaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
318
-
maltopentaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
341
-
maltotetraose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4