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Literature summary for 3.2.1.3 extracted from
- Luminal starch substrate "brake" on maltase-glucoamylase activity is located within the glucoamylase subunit (2008), J. Nutr., 138, 685-692.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acarbose | inhibitory effect is 2 orders of magnitude higher for ntMGAM than for native MGAM | Homo sapiens |  |
| maltose | - |
Homo sapiens | |
| maltotetraose | - |
Homo sapiens | |
| maltotriose | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value: 4.34 g/l with corn maltodextrin as a substrate for the N-terminal subunit of MGAM, Vmax: 7.51 U/mg with corn maltodextrin as a substrate for the N-terminal subunit of MGAM. Km-value: 7.01 g/l with alpha-limit dextrin as a substrate for the N-terminal subunit of MGAM, Vmax: 11.0 U/mg with alpha-limit dextrin as a substrate for the N-terminal subunit of MGAM. Km-value: 0.06 g/l with corn maltodextrin as a substrate for the immunoprecipitated maltase-glucoamylase. Km-value: 0.49 g/l with alpha-limit dextrin as a substrate for immunoprecipitated maltase-glucoamylase, Vmax: 11.0 U/mg with alpha-limit dextrin as a substrate for the immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 0.05 | - |
maltotetraose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 0.05 | - |
maltopentaose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 0.22 | - |
maltotriose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 0.58 | - |
maltose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3 | - |
maltotetraose | ntMGAM, N-terminal subunit of MGAM, Vmax: 7.65 U/mg | Homo sapiens | |
| 4.6 | - |
maltotriose | ntMGAM, N-terminal subunit of MGAM, Vmax: 9.58 U/mg | Homo sapiens | |
| 6.66 | - |
maltopentaose | ntMGAM, N-terminal subunit of MGAM, Vmax: 6.97 U/mg | Homo sapiens | |
| 7.71 | - |
maltose | ntMGAM, N-terminal subunit of MGAM, Vmax: 8.26 U/mg | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | analysis of the basic catalytic properties of the N-terminal subunit of MGAM (ntMGAM) on the hydrolysis of glucan substrates and compared it with those of human native MGAM | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-limit dextrin + H2O | - |
Homo sapiens | ? | - |
? | |
| maltodextrin + H2O | corn maltodextrin | Homo sapiens | ? | - |
? | |
| maltopentaose + H2O | - |
Homo sapiens | ? | - |
? | |
| maltose + H2O | - |
Homo sapiens | ? | - |
? | |
| maltotetraose + H2O | - |
Homo sapiens | ? | - |
? | |
| maltotriose + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | analysis of the basic catalytic properties of the N-terminal subunit of MGAM (ntMGAM) on the hydrolysis of glucan substrates and compared it with those of human native MGAM | Homo sapiens | ? | - |
? | |
| starch + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Homo sapiens |
| maltase-glucoamylase | - |
Homo sapiens |
| MGAM | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki-value: 12.7 g/l with corn maltodextrin as a substrate for the immunoprecipitated maltase-glucoamylase. Ki-value: 37.6 g/l with alpha-limit dextrin as a substrate for immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 0.000074 | - |
acarbose | IP-MGAM is highly sensitive to acarbose with a calculated Ki of 74 nmol/l | Homo sapiens | |
| 0.24 | - |
maltotriose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 0.58 | - |
maltotetraose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 16 | - |
acarbose | ntMGAM had a relatively low sensitivity to acarbose | Homo sapiens | |
| 35 | - |
maltotriose | ntMGAM, N-terminal subunit of MGAM | Homo sapiens | |
| 67.6 | - |
maltotetraose | ntMGAM, N-terminal subunit of MGAM | Homo sapiens | |
| 86.9 | - |
maltose | ntMGAM, N-terminal subunit of MGAM | Homo sapiens | |
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