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Literature summary for 3.2.1.3 extracted from

  • Peres, M.F.; Souza, C.S.; Thomaz, D.; de Souza, A.R.; Laluce, C.
    Partitioning of the glucoamylase activity at the cell surfaces in cultures of Saccharomyces (2006), Process Biochem., 41, 20-27.
No PubMed abstract available

Application

Application Comment Organism
industry glucoamylases have been used with alpha-amylases for the industrial conversion of starch into glucose, intact cells of thermotolerant yeasts can be used as colloidal biocatalysts in starch degradation processes Saccharomyces cerevisiae
industry glucoamylases have been used with alpha-amylases for the industrial conversion of starch into glucose, intact cells of thermotolerant yeasts can be used as colloidal biocatalysts in starch degradation processes Saccharomyces cerevisiae 'var. diastaticus'

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the extracellular enzyme is attached to the cell wall Saccharomyces cerevisiae 9986
-
cell surface the extracellular enzyme is attached to the cell wall Saccharomyces cerevisiae 'var. diastaticus' 9986
-
extracellular secretion Saccharomyces cerevisiae
-
-
extracellular secretion Saccharomyces cerevisiae 'var. diastaticus'
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Saccharomyces cerevisiae the enzyme plays a role in the saccharification and fermentation of amylaceous substrates, notably in high cell density processes D-glucose + ?
-
?
starch + H2O Saccharomyces cerevisiae 'var. diastaticus' the enzyme plays a role in the saccharification and fermentation of amylaceous substrates, notably in high cell density processes D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
strains DI-10, DI-11, and DI-20
-
Saccharomyces cerevisiae 'var. diastaticus'
-
hybrid strain CL-9
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Saccharomyces cerevisiae
glycoprotein
-
Saccharomyces cerevisiae 'var. diastaticus'

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cell culture growth conditions, optimization, partition factors, overview Saccharomyces cerevisiae
-
cell culture cell culture growth conditions, optimization, partition factors, overview Saccharomyces cerevisiae 'var. diastaticus'
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O soluble starch Saccharomyces cerevisiae D-glucose + ?
-
?
starch + H2O soluble starch Saccharomyces cerevisiae 'var. diastaticus' D-glucose + ?
-
?
starch + H2O the enzyme plays a role in the saccharification and fermentation of amylaceous substrates, notably in high cell density processes Saccharomyces cerevisiae D-glucose + ?
-
?
starch + H2O the enzyme plays a role in the saccharification and fermentation of amylaceous substrates, notably in high cell density processes Saccharomyces cerevisiae 'var. diastaticus' D-glucose + ?
-
?

Synonyms

Synonyms Comment Organism
glucoamylase
-
Saccharomyces cerevisiae
glucoamylase
-
Saccharomyces cerevisiae 'var. diastaticus'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
Saccharomyces cerevisiae
50 60
-
Saccharomyces cerevisiae 'var. diastaticus'

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
Saccharomyces cerevisiae
30 70
-
Saccharomyces cerevisiae 'var. diastaticus'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
Saccharomyces cerevisiae
4 4.5
-
Saccharomyces cerevisiae 'var. diastaticus'

pH Range

pH Minimum pH Maximum Comment Organism
2 8
-
Saccharomyces cerevisiae
2 8
-
Saccharomyces cerevisiae 'var. diastaticus'