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Literature summary for 3.2.1.3 extracted from
- Cloning of a gene encoding thermostable glucoamylase from Chaetomium thermophilum and its expression in Pichia pastoris (2007), J. Appl. Microbiol., 103, 2277-2284.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gla, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional overexpression in Pichia pastoris strain GS115, secretion to the culture supernatant | Thermochaetoides thermophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, recombinant enzyme, SDS-PAGE | Thermochaetoides thermophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides thermophila | Q0H9W2 | a soil-borne thermophilic fungus, gene gla | - |
| Thermochaetoides thermophila CT2 | Q0H9W2 | a soil-borne thermophilic fungus, gene gla | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Thermochaetoides thermophila |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris strain GS115 culture supernatant | Thermochaetoides thermophila |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Thermochaetoides thermophila | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.73 | - |
recombinant enzyme in Pichia pastoris | Thermochaetoides thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | soluble starch | Thermochaetoides thermophila | beta-D-glucose + ? | - |
? |  |
| starch + H2O | soluble starch | Thermochaetoides thermophila CT2 | beta-D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, recombinant enzyme, SDS-PAGE | Thermochaetoides thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermochaetoides thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant enzyme, stable | Thermochaetoides thermophila |
| 70 | - |
purified recombinant enzyme, 60 min, 80% remaining activity | Thermochaetoides thermophila |
| 80 | - |
purified recombinant enzyme, half-life is 40 min | Thermochaetoides thermophila |
| 90 | - |
purified recombinant enzyme, half-life is 10 min | Thermochaetoides thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Thermochaetoides thermophila |
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Release 2023.1 (January 2023)
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