General Stability | Organism |
---|---|
CaCl2 at 1 mM stabilizes the purified native enzyme | Aureobasidium pullulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Aureobasidium pullulans | |
EDTA | - |
Aureobasidium pullulans | |
EGTA | - |
Aureobasidium pullulans | |
Hg2+ | - |
Aureobasidium pullulans | |
additional information | no inhibition by iodoacetic acid and PMSF | Aureobasidium pullulans | |
SDS | - |
Aureobasidium pullulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.75 | - |
soluble starch | pH 4.5, 60°C | Aureobasidium pullulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aureobasidium pullulans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Aureobasidium pullulans | |
Ca2+ | activates | Aureobasidium pullulans | |
Co2+ | activates | Aureobasidium pullulans | |
Cu2+ | activates | Aureobasidium pullulans | |
Mg2+ | activates | Aureobasidium pullulans | |
Na+ | activates | Aureobasidium pullulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 65000 + 1 * 33000, SDS-PAGE | Aureobasidium pullulans |
65000 | - |
1 * 65000 + 1 * 33000, SDS-PAGE | Aureobasidium pullulans |
98000 | - |
native extracellular enzyme, native PAGE | Aureobasidium pullulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Aureobasidium pullulans | - |
D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aureobasidium pullulans | - |
marine yeast, strain N13d isolated from deep sea of Pacific Ocean | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from culture supernatant 7.3fold to homogeneity by ammonium sulfate fractionation and gel filtration | Aureobasidium pullulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Aureobasidium pullulans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
297.3 | - |
purified native enzyme | Aureobasidium pullulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has debranching activity | Aureobasidium pullulans | ? | - |
? | |
soluble starch + H2O | - |
Aureobasidium pullulans | beta-D-glucose + soluble starch | - |
? | |
starch + H2O | - |
Aureobasidium pullulans | D-glucose + ? | - |
? | |
starch + H2O | raw and cooked soluble starch from potato, no activity with corn starch and sweet potato starch | Aureobasidium pullulans | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 65000 + 1 * 33000, SDS-PAGE | Aureobasidium pullulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aureobasidium pullulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aureobasidium pullulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified native enzyme, partial inactivation | Aureobasidium pullulans |
4 | 8 | purified native enzyme, stable, over 75% remaining activity after 3 h at 55°C | Aureobasidium pullulans |
9 | - |
purified native enzyme, partial inactivation | Aureobasidium pullulans |