Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.3 extracted from

  • Li, H.; Chi, Z.; Wang, X.; Duan, X.; Ma, L.; Gao, L.
    Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion (2007), Enzyme Microb. Technol., 40, 1006-1012.
No PubMed abstract available

General Stability

General Stability Organism
CaCl2 at 1 mM stabilizes the purified native enzyme Aureobasidium pullulans

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Aureobasidium pullulans
EDTA
-
Aureobasidium pullulans
EGTA
-
Aureobasidium pullulans
Hg2+
-
Aureobasidium pullulans
additional information no inhibition by iodoacetic acid and PMSF Aureobasidium pullulans
SDS
-
Aureobasidium pullulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.75
-
soluble starch pH 4.5, 60°C Aureobasidium pullulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aureobasidium pullulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Aureobasidium pullulans
Ca2+ activates Aureobasidium pullulans
Co2+ activates Aureobasidium pullulans
Cu2+ activates Aureobasidium pullulans
Mg2+ activates Aureobasidium pullulans
Na+ activates Aureobasidium pullulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
1 * 65000 + 1 * 33000, SDS-PAGE Aureobasidium pullulans
65000
-
1 * 65000 + 1 * 33000, SDS-PAGE Aureobasidium pullulans
98000
-
native extracellular enzyme, native PAGE Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Aureobasidium pullulans
-
D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aureobasidium pullulans
-
marine yeast, strain N13d isolated from deep sea of Pacific Ocean
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from culture supernatant 7.3fold to homogeneity by ammonium sulfate fractionation and gel filtration Aureobasidium pullulans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aureobasidium pullulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
297.3
-
purified native enzyme Aureobasidium pullulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has debranching activity Aureobasidium pullulans ?
-
?
soluble starch + H2O
-
Aureobasidium pullulans beta-D-glucose + soluble starch
-
?
starch + H2O
-
Aureobasidium pullulans D-glucose + ?
-
?
starch + H2O raw and cooked soluble starch from potato, no activity with corn starch and sweet potato starch Aureobasidium pullulans D-glucose + ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 65000 + 1 * 33000, SDS-PAGE Aureobasidium pullulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aureobasidium pullulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aureobasidium pullulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
purified native enzyme, partial inactivation Aureobasidium pullulans
4 8 purified native enzyme, stable, over 75% remaining activity after 3 h at 55°C Aureobasidium pullulans
9
-
purified native enzyme, partial inactivation Aureobasidium pullulans