Cloned (Comment) | Organism |
---|---|
phylogenetic tree, expression of the intracellular enzyme in Escherichia coli | Picrophilus torridus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
maltotriose | pH 5.0, 50°C, recombinant enzyme | Picrophilus torridus | |
4.9 | - |
maltose | pH 5.0, 50°C, recombinant enzyme | Picrophilus torridus | |
9.9 | - |
maltotetraose | pH 5.0, 50°C, recombinant enzyme | Picrophilus torridus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Picrophilus torridus | - |
- |
intracellular | - |
Picrophilus torridus | 5622 | - |
additional information | the extracellular enzyme contains a signal sequence | Picrophilus torridus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
4 * 73000, intracellular enzyme, SDS-PAGE | Picrophilus torridus |
92000 | - |
x * 92000, extracellular enzyme | Picrophilus torridus |
292000 | - |
recombinant intracellular enzyme, native PAGE | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6KYX5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the extracellular enzyme contains a signal sequence | Picrophilus torridus |
Purification (Comment) | Organism |
---|---|
recombinant intracellular enzyme 37fold from Escherichia coli by heat treatment at 70°C for 30 min, anion exchange chromatography, and gel filtration to homogeneity | Picrophilus torridus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | optimal growth at pH 0.7 and 60 °C | Picrophilus torridus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | potato amylopectin | Picrophilus torridus | D-glucose + ? | - |
? | |
amylose + H2O | potato amylose type III | Picrophilus torridus | D-glucose + ? | - |
? | |
dextrin + 6 H2O | - |
Picrophilus torridus | 7 D-glucose | - |
? | |
maltose + H2O | - |
Picrophilus torridus | 2 D-glucose | - |
? | |
maltotetraose + H2O | - |
Picrophilus torridus | maltotriose + D-glucose | - |
? | |
maltotriose + H2O | - |
Picrophilus torridus | maltose + D-glucose | - |
? | |
additional information | the intracellular enzyme shows a unique substrate specificity compared to already known glucoamylases, in addition to the hydrolysis of branched and linear alpha-glucans, the purified enzyme preferentially attacks maltotriose, overview, oligossaccharides Dp2, Dp4, Dp5, Dp6, and Dp7 as well as isomaltose, panose, and isopanose are poor substrates | Picrophilus torridus | ? | - |
? | |
starch + H2O | - |
Picrophilus torridus | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000, extracellular enzyme | Picrophilus torridus |
tetramer | 4 * 73000, intracellular enzyme, SDS-PAGE | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
intracellular enzyme | Picrophilus torridus |
90 | - |
extracellular enzyme | Picrophilus torridus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | intracellular enzyme | Picrophilus torridus |
50 | 100 | extracellular enzyme | Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant intracellular enzyme, 48 h, stable | Picrophilus torridus |
55 | - |
purified recombinant intracellular enzyme, half-life is 8 h | Picrophilus torridus |
60 | - |
purified recombinant intracellular enzyme, half-life is 1 h | Picrophilus torridus |
65 | - |
purified recombinant intracellular enzyme, inctivation within 5 min | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
extracellular enzyme | Picrophilus torridus |
5 | - |
intracellular enzyme | Picrophilus torridus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
- |
4 | extracellular enzyme | Picrophilus torridus |
4.5 | 6.5 | intracellular enzyme | Picrophilus torridus |