Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is industrially an important biocatalyst that decomposes starch into glucose by tearing-off alpha-1,4-linked glucose residue from the non-reduced end of the polysaccharide chain | Curvularia lunata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Curvularia lunata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 5.0fold by ammonium sulfate fractionation, gel filtration, and cation and anion exchange chromatography to homogeneity | Curvularia lunata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.54 | - |
culture supernatant, substrate starch | Curvularia lunata |
19.73 | - |
purified enzyme, substrate starch | Curvularia lunata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the glucoamylase also shows steroidal saponin-rhamnosidase activity, EC 3.2.1.40, being able to hydrolyze the terminal rhamnosyl of steroidal saponins and the sugar chain at the C-3 position of spirostanosides, the enzyme also hydrolyzed the terminal rhamnosyl residues of the sugar chain at the C-3 position while retaining the glucosyl residues at the C-26 position of furostanosides, substrate specificity, overview | Curvularia lunata | ? | - |
? | |
starch + H2O | soluble starch | Curvularia lunata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Curvularia lunata |
More | enzyme amino acid sequence analysis by MALDI-TOF mass spectrometry | Curvularia lunata |
Synonyms | Comment | Organism |
---|---|---|
1,4-alpha-D-glucan glucohydrolase | - |
Curvularia lunata |
glucoamylase | - |
Curvularia lunata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Curvularia lunata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Curvularia lunata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme, 30-40% remaining activity | Curvularia lunata |
30 | - |
purified enzyme, stable | Curvularia lunata |
30 | 40 | purified enzyme, 80% of maximal activity within this range | Curvularia lunata |
50 | - |
purified enzyme, 70% remaining activity | Curvularia lunata |
70 | - |
purified enzyme, 30-40% remaining activity | Curvularia lunata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Curvularia lunata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Curvularia lunata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified enzyme, 60% remaining activity | Curvularia lunata |
4 | 7 | purified enzyme, 90% of maximal activity within this range | Curvularia lunata |