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Literature summary for 3.2.1.3 extracted from

  • Tsao, C.; Hsu, Y.; Chao, L.; Jiang, S.
    Purification and characterization of three amylases from viscera of hard clam Meretrix lusoria (2004), Fish. Sci., 70, 174-182.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
Meretrix lusoria
Ba2+ slight inhibition Meretrix lusoria
Cd2+ complete inhibition Meretrix lusoria
Co2+
-
Meretrix lusoria
Cu2+ strong inhibition Meretrix lusoria
EDTA strong inhibition Meretrix lusoria
Fe3+ complete inhibition Meretrix lusoria
Hg2+ complete inhibition Meretrix lusoria
additional information iodoacetamide and urea are poor inhibitors Meretrix lusoria
NEM slight inhibition Meretrix lusoria
Ni2+ strong inhibition Meretrix lusoria
PMSF slight inhibition Meretrix lusoria
Sr2+ slight inhibition Meretrix lusoria
Zn2+ strong inhibition Meretrix lusoria

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Meretrix lusoria
additional information no requirement for Ca2+, the enzyme is not affected by K+, and Mg2+ Meretrix lusoria
Na+ activates Meretrix lusoria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
x * 100000, SDS-PAGE Meretrix lusoria

Organism

Organism UniProt Comment Textmining
Meretrix lusoria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 18fold from viscus, by ammonium sulfate fractionation, alternating gel filtration and ion exchange chromatography steps, to homogeneity Meretrix lusoria

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
Meretrix lusoria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.8
-
purified enzyme Meretrix lusoria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O exoglycosidic hydrolysis of terminal glucose, best substrate Meretrix lusoria amylopectin + beta-D-glucose
-
?
amylose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria amylose + beta-D-glucose
-
?
glycogen + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria glycogen + beta-D-glucose
-
?
maltopentaose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria maltotetraose + beta-D-glucose
-
?
maltotetraose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria maltotriose + beta-D-glucose
-
?
maltotriose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria maltose + beta-D-glucose
-
?
additional information no activity with cyclodextrins and laminarin Meretrix lusoria ?
-
?
pullulan + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria pullulan + beta-D-glucose
-
?
starch + H2O exoglycosidic hydrolysis of terminal glucose, high activity Meretrix lusoria starch + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Meretrix lusoria

Synonyms

Synonyms Comment Organism
exo-amylase
-
Meretrix lusoria
gamma-amylase
-
Meretrix lusoria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Meretrix lusoria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Meretrix lusoria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Meretrix lusoria