Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | - |
Meretrix lusoria | |
Ba2+ | slight inhibition | Meretrix lusoria | |
Cd2+ | complete inhibition | Meretrix lusoria | |
Co2+ | - |
Meretrix lusoria | |
Cu2+ | strong inhibition | Meretrix lusoria | |
EDTA | strong inhibition | Meretrix lusoria | |
Fe3+ | complete inhibition | Meretrix lusoria | |
Hg2+ | complete inhibition | Meretrix lusoria | |
additional information | iodoacetamide and urea are poor inhibitors | Meretrix lusoria | |
NEM | slight inhibition | Meretrix lusoria | |
Ni2+ | strong inhibition | Meretrix lusoria | |
PMSF | slight inhibition | Meretrix lusoria | |
Sr2+ | slight inhibition | Meretrix lusoria | |
Zn2+ | strong inhibition | Meretrix lusoria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Meretrix lusoria | |
additional information | no requirement for Ca2+, the enzyme is not affected by K+, and Mg2+ | Meretrix lusoria | |
Na+ | activates | Meretrix lusoria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, SDS-PAGE | Meretrix lusoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meretrix lusoria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 18fold from viscus, by ammonium sulfate fractionation, alternating gel filtration and ion exchange chromatography steps, to homogeneity | Meretrix lusoria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
viscus | - |
Meretrix lusoria | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46.8 | - |
purified enzyme | Meretrix lusoria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | exoglycosidic hydrolysis of terminal glucose, best substrate | Meretrix lusoria | amylopectin + beta-D-glucose | - |
? | |
amylose + H2O | exoglycosidic hydrolysis of terminal glucose | Meretrix lusoria | amylose + beta-D-glucose | - |
? | |
glycogen + H2O | exoglycosidic hydrolysis of terminal glucose | Meretrix lusoria | glycogen + beta-D-glucose | - |
? | |
maltopentaose + H2O | exoglycosidic hydrolysis of terminal glucose | Meretrix lusoria | maltotetraose + beta-D-glucose | - |
? | |
maltotetraose + H2O | exoglycosidic hydrolysis of terminal glucose | Meretrix lusoria | maltotriose + beta-D-glucose | - |
? | |
maltotriose + H2O | exoglycosidic hydrolysis of terminal glucose | Meretrix lusoria | maltose + beta-D-glucose | - |
? | |
additional information | no activity with cyclodextrins and laminarin | Meretrix lusoria | ? | - |
? | |
pullulan + H2O | exoglycosidic hydrolysis of terminal glucose | Meretrix lusoria | pullulan + beta-D-glucose | - |
? | |
starch + H2O | exoglycosidic hydrolysis of terminal glucose, high activity | Meretrix lusoria | starch + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Meretrix lusoria |
Synonyms | Comment | Organism |
---|---|---|
exo-amylase | - |
Meretrix lusoria |
gamma-amylase | - |
Meretrix lusoria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Meretrix lusoria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Meretrix lusoria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Meretrix lusoria |