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Literature summary for 3.2.1.3 extracted from

  • Rey, M.W.; Brown, K.M.; Golightly, E.J.; Fuglsang, C.C.; Nielsen, B.R.; Hendriksen, H.V.; Butterworth, A.; Xu, F.
    Cloning, heterologous expression, and characterization of Thielavia terrestris glucoamylase (2003), Appl. Biochem. Biotechnol., 111, 153-166.
    View publication on PubMed
Search for more literature for 3.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Thermothielavioides terrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 maltotriose 20°C, pH 4.0 Thermothielavioides terrestris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, deduced from nucleotide sequence Thermothielavioides terrestris
75000
-
 x * 75000, SDS-PAGE Thermothielavioides terrestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltodextrin + H2O Thermothielavioides terrestris
-
 beta-D-glucose + ?
-
 ?
maltose + H2O Thermothielavioides terrestris
-
 beta-D-glucose + D-glucose
-
 ?
maltotriose + 2 H2O Thermothielavioides terrestris
-
 3 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris
-
 soil-borne thermophilic fungus, synonym, allescheria terrestris
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein five potential sites for N-glycosylation Thermothielavioides terrestris

Purification (Commentary)

Purification (Comment) Organism
recombinant tGA Thermothielavioides terrestris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 with maltose Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + H2O
-
 Thermothielavioides terrestris beta-D-glucose + ?
-
 ?
maltose + H2O
-
 Thermothielavioides terrestris beta-D-glucose + D-glucose
-
 ?
maltotriose + 2 H2O
-
 Thermothielavioides terrestris 3 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Thermothielavioides terrestris
? x * 62000, deduced from nucleotide sequence Thermothielavioides terrestris

Synonyms

Synonyms Comment Organism
tGA
-
 Thermothielavioides terrestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 with maltotriose Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 no loss of activity after 30 min Thermothielavioides terrestris
70
-
 30-40% loss of activity after 10 min, approx. 90% loss of activity after 40 min, complete loss of activity after 60 min Thermothielavioides terrestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
91.7
-
 maltotriose 20°C, pH 4.0 Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 with maltotriose Thermothielavioides terrestris

pH Range

pH Minimum pH Maximum Comment Organism
3 8 approx. 70% of maximal activity at pH 4.0, approx. 5% of maximal activity at pH 8.0 Thermothielavioides terrestris