Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris, Saccharomyces cerevisiae or Aspergillus niger. In Pichia pastoris the C320A/E400C double mutant is produced at 75% of the wild-type glucoamylase, while the corresponding single mutants are produced at 1% and 20% of the wild-type level, respectively | Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
maltotriose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
0.28 | - |
maltotriose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
0.29 | - |
maltotriose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori | |
1.21 | - |
maltose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
1.82 | - |
maltose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
2.97 | - |
maltose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80370 | - |
MALDI-MS | Aspergillus awamori |
82330 | - |
recombinant enzyme expressed in Pichia pastoris, matrix-assisted laser desorption ionization mass spectrometry | Aspergillus awamori |
82840 | - |
recombinant enzyme expressed in Aspergillus niger, MALDI-MS | Aspergillus awamori |
83870 | - |
recombinant enzyme expressed in Saccharomyces cerevisiae, matrix-assisted laser desorption ionization mass spectrometry | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
wild-type and mutant enzyme C320A/E400C | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltotriose + H2O | - |
Aspergillus awamori | maltose + glucose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers for the recombinant enzymes with maltose through maltoheptaose as substrates | Aspergillus awamori | |
6.8 | - |
maltose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori | |
10.7 | - |
maltose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
11.3 | - |
maltose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
27.6 | - |
maltotriose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori | |
30.9 | - |
maltotriose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
33.5 | - |
maltotriose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
44.7 | - |
maltotetraose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori |