Any feedback?
Literature summary for 3.2.1.3 extracted from
- Purification and properties of the raw-starch-digesting glucoamylases from Corticium rolfsii (1998), Appl. Microbiol. Biotechnol., 50, 323-330.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
starch | soluble, glucoamylase G1 or G3 | Athelia rolfsii |  |
| 0.0002 | - |
amylopectin | glucoamylase G2 or G3 | Athelia rolfsii | |
| 0.0003 | - |
starch | soluble, glucoamylase G2 | Athelia rolfsii | |
| 0.0003 | - |
amylopectin | glucoamylase G1 | Athelia rolfsii | |
| 0.0005 | - |
glycogen | glucoamylase G2 | Athelia rolfsii | |
| 0.0006 | - |
glycogen | glucoamylase G3 | Athelia rolfsii | |
| 0.0007 | - |
glycogen | glucoamylase G1 | Athelia rolfsii | |
| 0.0021 | - |
amylose | DP = 18, glucoamylase G1, G2 or G3 | Athelia rolfsii | |
| 0.025 | - |
maltoheptaose | glucoamylase G2 | Athelia rolfsii | |
| 0.026 | - |
maltoheptaose | glucoamylase G1 | Athelia rolfsii | |
| 0.027 | - |
maltoheptaose | glucoamylase G3 | Athelia rolfsii | |
| 0.045 | - |
maltohexaose | glucoamylase G2 | Athelia rolfsii | |
| 0.045 | - |
starch | soluble, glucoamylase G4 | Athelia rolfsii | |
| 0.048 | - |
maltohexaose | glucoamylase G1 | Athelia rolfsii | |
| 0.048 | - |
starch | soluble, glucoamylase G5 | Athelia rolfsii | |
| 0.05 | - |
maltohexaose | glucoamylase G3 | Athelia rolfsii | |
| 0.058 | - |
maltoheptaose | glucoamylase G5 | Athelia rolfsii | |
| 0.059 | - |
maltohexaose | glucoamylase G4 | Athelia rolfsii | |
| 0.059 | - |
maltoheptaose | glucoamylase G4 | Athelia rolfsii | |
| 0.06 | - |
maltohexaose | glucoamylase G5 | Athelia rolfsii | |
| 0.063 | - |
amylose DP 18 | glucoamylase G4 | Athelia rolfsii | |
| 0.065 | - |
amylose DP 18 | glucoamylase G5 | Athelia rolfsii | |
| 0.066 | - |
maltopentaose | glucoamylase G3 | Athelia rolfsii | |
| 0.067 | - |
maltopentaose | glucoamylase G4 or G5 | Athelia rolfsii | |
| 0.07 | - |
maltopentaose | glucoamylase G1 | Athelia rolfsii | |
| 0.071 | - |
maltopentaose | glucoamylase G2 | Athelia rolfsii | |
| 0.082 | - |
maltotetraose | glucoamylase G4 | Athelia rolfsii | |
| 0.083 | - |
maltotetraose | glucoamylase G5 | Athelia rolfsii | |
| 0.088 | - |
maltotetraose | glucoamylase G3 | Athelia rolfsii | |
| 0.095 | - |
maltotetraose | glucoamylase G1 or G2 | Athelia rolfsii | |
| 0.16 | - |
amylopectin | glucoamylase G5 | Athelia rolfsii | |
| 0.17 | - |
amylopectin | glucoamylase G4 | Athelia rolfsii | |
| 0.21 | - |
maltotriose | glucoamylase G1, G2 or G3 | Athelia rolfsii | |
| 0.23 | - |
maltotriose | glucoamylase G4 | Athelia rolfsii | |
| 0.24 | - |
maltotriose | glucoamylase G5 | Athelia rolfsii | |
| 0.62 | - |
glycogen | glucoamylase G5 | Athelia rolfsii | |
| 0.63 | - |
glycogen | glucoamylase G4 | Athelia rolfsii | |
| 0.67 | - |
maltose | glucoamylase G4 and G5 | Athelia rolfsii | |
| 0.68 | - |
maltose | glucoamylase G1 and G2 | Athelia rolfsii | |
| 0.69 | - |
maltose | glucoamylase G3 | Athelia rolfsii | |
| 15.4 | - |
panose | glucoamylase G1 | Athelia rolfsii | |
| 15.8 | - |
panose | glucoamylase G2 | Athelia rolfsii | |
| 16.7 | - |
panose | glucoamylase G4 | Athelia rolfsii | |
| 16.9 | - |
panose | glucoamylase G5 | Athelia rolfsii | |
| 17.2 | - |
panose | glucoamylase G3 | Athelia rolfsii | |
| 20 | - |
isomaltose | glucoamylase G1 | Athelia rolfsii | |
| 20.1 | - |
isomaltose | glucoamylase G2 | Athelia rolfsii | |
| 20.4 | - |
isomaltose | glucoamylase G3 | Athelia rolfsii | |
| 21.7 | - |
isomaltose | glucoamylase G4 | Athelia rolfsii | |
| 23 | - |
isomaltose | glucoamylase G5 | Athelia rolfsii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, glucoamylase G5, SDS-PAGE | Athelia rolfsii |
| 58000 | - |
x * 58000, glucoamylase G4, SDS-PAGE | Athelia rolfsii |
| 69000 | - |
1 * 69000, glucoamylase G5, SDS-PAGE | Athelia rolfsii |
| 70000 | - |
1 * 70000, glucoamylase G4, SDS-PAGE | Athelia rolfsii |
| 72000 | - |
x * 72000, glucoamylase 2, SDS-PAGE | Athelia rolfsii |
| 73000 | - |
x * 73000, glucoamylase 1, SDS-PAGE | Athelia rolfsii |
| 74000 | - |
glucoamylase G3, SDS-PAGE | Athelia rolfsii |
| 78000 | - |
1 * 78000, glucoamylase 1 and 2, SDS-PAGE | Athelia rolfsii |
| 79000 | - |
1 * 79000, glucoamylase G3, SDS-PAGE | Athelia rolfsii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Athelia rolfsii | - |
AHU 9627 | - |
| Athelia rolfsii AHU 9627 | - |
AHU 9627 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glucoamylase G1 contains 8.1% carbohydrate, glucoamylase G2 contains 7.6% carbohydrate, glucoamylase G3 contains 8.0% carbohydrate, glucoamylase G4 and G5 contain 7.5% carbohydrate | Athelia rolfsii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 5 forms of glucoamylase: G1-G5 | Athelia rolfsii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29 | - |
enzyme form G4 | Athelia rolfsii |
| 38 | - |
enzyme form G3 | Athelia rolfsii |
| 39 | - |
enzyme form G2 | Athelia rolfsii |
| 41 | - |
enzyme form G1 | Athelia rolfsii |
| 44 | - |
enzyme form G4 | Athelia rolfsii |
| 47 | - |
enzyme form G5 | Athelia rolfsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Athelia rolfsii | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Athelia rolfsii AHU 9627 | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Athelia rolfsii | glucose + ? | - |
? | |
| amylose + H2O | - |
Athelia rolfsii AHU 9627 | glucose + ? | - |
? | |
| amylose DP 18 + H2O | - |
Athelia rolfsii | beta-D-glucose + amylose DP 17 | - |
? | |
| glycogen + H2O | - |
Athelia rolfsii | glucose + ? | - |
? | |
| glycogen + H2O | - |
Athelia rolfsii AHU 9627 | glucose + ? | - |
? | |
| isomaltose + H2O | - |
Athelia rolfsii | glucose | - |
? | |
| maltoheptaose + H2O | - |
Athelia rolfsii | ? | - |
? | |
| maltohexaose + H2O | - |
Athelia rolfsii | ? | - |
? | |
| maltopentaose + H2O | - |
Athelia rolfsii | ? | - |
? | |
| maltose + H2O | - |
Athelia rolfsii | 2 glucose | - |
? | |
| maltotetraose + H2O | - |
Athelia rolfsii | ? | - |
? | |
| maltotriose + H2O | - |
Athelia rolfsii | maltose + glucose | - |
? | |
| panose + H2O | - |
Athelia rolfsii | ? | - |
? | |
| starch + H2O | soluble | Athelia rolfsii | glucose + ? | - |
? | |
| starch + H2O | raw starch and gelatinized starch | Athelia rolfsii | glucose + ? | - |
? | |
| starch + H2O | soluble | Athelia rolfsii AHU 9627 | glucose + ? | - |
? | |
| starch + H2O | raw starch and gelatinized starch | Athelia rolfsii AHU 9627 | glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, glucoamylase 2, SDS-PAGE | Athelia rolfsii |
| ? | x * 74000, glucoamylase G3, SDS-PAGE | Athelia rolfsii |
| ? | x * 73000, glucoamylase 1, SDS-PAGE | Athelia rolfsii |
| ? | x * 58000, glucoamylase G4, SDS-PAGE | Athelia rolfsii |
| ? | x * 57000, glucoamylase G5, SDS-PAGE | Athelia rolfsii |
| monomer | 1 * 79000, glucoamylase G3, SDS-PAGE | Athelia rolfsii |
| monomer | 1 * 78000, glucoamylase 1 and 2, SDS-PAGE | Athelia rolfsii |
| monomer | 1 * 69000, glucoamylase G5, SDS-PAGE | Athelia rolfsii |
| monomer | 1 * 70000, glucoamylase G4, SDS-PAGE | Athelia rolfsii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
hydrolysis of raw starch, enzyme form G1, G2, and G3 | Athelia rolfsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, pH 4.0, enzyme form G1, G2, G3, G4 and G5, stable up to | Athelia rolfsii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | 30°C, 24 h, stable | Athelia rolfsii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
