Protein Variants | Comment | Organism |
---|---|---|
S411A | 54-74% of the catalytic efficiency of the wild type enzyme. Increased pH-optimum by 0.8 units for both maltose and maltoheptaose hydrolysis while maintaining a high level of activity and catalytic efficiency. In hydrolysis of 28% DE 10 maltodextrin, the mutant enzyme has a pH optimum of 7 compared with 5.6 for wild-type enzyme, and has higher initial rates of glucose production than wild-type enzyme at all pH values tested above pH 6.6 | Aspergillus awamori |
S411C | 54-74% of the catalytic efficiency of the wild type enzyme | Aspergillus awamori |
S411D | 6-12% of the catalytic efficiency of the wild type enzyme | Aspergillus awamori |
S411G | catalytic efficiency like that of wild type enzyme for isomaltose, maltose and maltoheptaose hydrolysis at pH 4.4 | Aspergillus awamori |
S411H | 6-12% of the catalytic efficiency of the wild type enzyme | Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
maltoheptaose | mutant enzyme S411C | Aspergillus awamori | |
0.083 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
0.104 | - |
maltoheptaose | mutant enzyme S411A | Aspergillus awamori | |
0.132 | - |
maltoheptaose | mutant enzyme S411G | Aspergillus awamori | |
0.148 | - |
maltoheptaose | mutant enzyme S411D | Aspergillus awamori | |
0.53 | - |
maltose | mutant enzyme S411C | Aspergillus awamori | |
1.01 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
1.26 | - |
maltose | mutant enzyme S411A | Aspergillus awamori | |
1.59 | - |
maltose | mutant enzyme S411G | Aspergillus awamori | |
3.58 | - |
maltose | mutant enzyme S411D | Aspergillus awamori | |
12.3 | - |
isomaltose | mutant enzyme S411C | Aspergillus awamori | |
23.5 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
26.2 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
27.9 | - |
isomaltose | mutant enzyme S411A | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
isomaltose | mutant enzyme S411C | Aspergillus awamori | |
0.63 | - |
isomaltose | mutant enzyme S411A | Aspergillus awamori | |
0.72 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
0.93 | - |
isomaltose | mutant enzyme S411G | Aspergillus awamori | |
4.36 | - |
maltose | mutant enzyme S411D | Aspergillus awamori | |
7.78 | - |
maltose | mutant enzyme S411C | Aspergillus awamori | |
14.5 | - |
maltose | mutant enzyme S411G | Aspergillus awamori | |
15.8 | - |
maltoheptaose | mutant enzyme S411D | Aspergillus awamori | |
18.9 | - |
maltose | mutant enzyme S411A | Aspergillus awamori | |
20.4 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
33 | - |
maltoheptaose | mutant enzyme S411C | Aspergillus awamori | |
59.4 | - |
maltoheptaose | mutant enzyme S411A | Aspergillus awamori | |
72.3 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
84 | - |
maltoheptaose | mutant enzyme S411G | Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolysis of maltose, wild-type enzyme | Aspergillus awamori |
4.1 | - |
hydrolysis of maltose, mutant enzyme S411H | Aspergillus awamori |
4.2 | - |
hydrolysis of maltose, mutant enzyme S411G | Aspergillus awamori |
4.4 | - |
hydrolysis of maltose, mutant enzyme S411D | Aspergillus awamori |
4.7 | - |
hydrolysis of maltoheptaose, wild-type enzyme | Aspergillus awamori |
4.8 | - |
hydrolysis of maltose, mutant enzyme S411A and S411C | Aspergillus awamori |
5 | - |
hydrolysis of maltoheptaose, mutant enzyme S411G | Aspergillus awamori |
5.5 | - |
hydrolysis of maltoheptaose, mutant enzyme S411A | Aspergillus awamori |