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Literature summary for 3.2.1.3 extracted from
- Purification and properties of two forms of glucoamylase from Penicillium oxalicum (1977), Agric. Biol. Chem., 41, 755-762.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
glucoamylase I, calculation from diffusion and sedimentation data | Penicillium oxalicum |
| 86000 | - |
glucoamylase II, calculation from diffusion and sedimentation data | Penicillium oxalicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium oxalicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glucoamylase I and II | Penicillium oxalicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 377.4 | - |
glucoamylase I | Penicillium oxalicum |
| 457.5 | - |
glucoamylase II | Penicillium oxalicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | soluble | Penicillium oxalicum | glucose + ? | - |
? |  |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 70 | 45°C: about 35% of maximal activity, glucoamylase I, about 60% of maximal activity, glucoamylase II. 70°C: about 30% of maximal activity, glucoamylase I and II | Penicillium oxalicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
15 min, stable up to | Penicillium oxalicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
glucoamylase II | Penicillium oxalicum |
| 5 | - |
glucoamylase I | Penicillium oxalicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | pH 3.0: about 40% of maximal activity for glucoamylase II, about 70% of maximal activity for glucoamylase I. pH 6.0: about 75% of maximal activity for glucoamylase I and II | Penicillium oxalicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | 30°C, 20 h, stable | Penicillium oxalicum |
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Release 2023.1 (January 2023)
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