Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Mucor rouxianus | |
Pb2+ | - |
Mucor rouxianus | |
Tris | - |
Mucor rouxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.741 mg/ml, hydrolysis of amylopectin with glucoamylase I. 0.667 mg/ml, hydrolysis of amylopectin with glucoamylase II. 0.455 mg/ml. Hydrolysis of amylose with glucoamylase I. 0.286 mg/ml, hydrolysis of amylose with glucoamylase II. 1.11 mg/ml, hydrolysis of glycogen with glucoamylase I. 1.54 mg/ml, hydrolysis of glycogen with glucoamylase II | Mucor rouxianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor rouxianus | - |
2 enzyme forms: glucoamylase I and II | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2.74% neutral sugar | Mucor rouxianus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | wheat bran culture | Mucor rouxianus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Mucor rouxianus | D-glucose + ? | - |
? | |
amylose + H2O | - |
Mucor rouxianus | glucose + ? | beta-glucose | ? | |
glycogen + H2O | - |
Mucor rouxianus | glucose + ? | - |
? | |
maltose + H2O | - |
Mucor rouxianus | 2 glucose | - |
? | |
maltotriose + H2O | - |
Mucor rouxianus | maltose + glucose | - |
? | |
phenyl alpha-maltoside + H2O | - |
Mucor rouxianus | glucose + phenyl alpha-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Mucor rouxianus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | 40°C: about 65% of maximal activity, 60°C: about 50% of maximal activity, glucoamylase II, about 60% of maximal activity, amylase I | Mucor rouxianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 4.6, 15 min, stable up to | Mucor rouxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
glucoamylase I | Mucor rouxianus |
5 | - |
glucoamylase II | Mucor rouxianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 30°C, 24 h, glucoamylase I, stable | Mucor rouxianus |
4 | 7.5 | 30°C, 24 h, glucoamylase II is stable | Mucor rouxianus |