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Literature summary for 3.2.1.3 extracted from
- Purification and properties of a thermophilic amyloglucosidase from Aspergillus niger (1983), Eur. J. Appl. Microbiol. Biotechnol., 18, 271-278.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | stimulates at 1 mM or at 10 mM | Aspergillus niger |  |
| iodoacetamide | stimulates | Aspergillus niger | |
| Schardinger dextrin | slight stimulation of activity with maltose as substrate | Aspergillus niger | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Aspergillus niger | |
| Cu2+ | - |
Aspergillus niger | |
| gentiobiose | 20 mM, uncompetitive inhibition with starch as substrate | Aspergillus niger | |
| maltitol | 20 mM, noncompetitive inhibition with starch as substrate | Aspergillus niger | |
| methyl alpha-D-glucoside | competitive with maltose and non-competitive with starch | Aspergillus niger | |
| Ni2+ | - |
Aspergillus niger | |
| p-hydroxymercuribenzoate | - |
Aspergillus niger | |
| Schardinger dextrin | mixed inhibition with starch | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.025% for starch | Aspergillus niger | |
| 1.42 | - |
maltotriose | - |
Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
| Ba2+ | 1 mM, some stimulation | Aspergillus niger | |
| Borate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
| Carbonate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
| diphosphate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
| phosphate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
| Sn2+ | 1 mM, some stimulation | Aspergillus niger | |
| Zn2+ | 1 mM, some stimulation | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | 31% of the activity with starch | Aspergillus niger | glucose + ? | - |
? | |
| maltose + H2O | - |
Aspergillus niger | 2 glucose | - |
? | |
| maltotriose + H2O | 68% of the activity with starch | Aspergillus niger | maltose + glucose | - |
? | |
| panose + H2O | 14% of the activity with starch | Aspergillus niger | ? | - |
? | |
| pullulan + H2O | 1.46% of the activity with starch | Aspergillus niger | ? | - |
? | |
| starch + H2O | with concentrated solutions of D-glucose, 10% w/v and 30% w/v, small amounts of isomaltose are produced as the sole reversion product | Aspergillus niger | glucose + ? | - |
? | |
| sucrose + H2O | 3.3% of carbohydrate | Aspergillus niger | D-glucose + D-fructose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 70 | - |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
starch or glycerol improve the thermal stability | Aspergillus niger |
| 50 | - |
pH 4.5, 30 min, stable | Aspergillus niger |
| 60 | - |
pH 4.5, 30 min, 10% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
with maltose or starch as substrate | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 6.5 | pH 1.5: about 70% of maximal activity with starch as substrate, about 60% of the activity with maltose as substrate, pH 6.5: about 15% of maximal activity with maltose as substrate, about 55% of maximal activity with starch as substrate | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
40°C, 30 min, 21% loss of activity | Aspergillus niger |
| 11 | - |
40°C, 30 min, 50% loss of activity | Aspergillus niger |
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