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Literature summary for 3.2.1.3 extracted from
- Fungal glucoamylases (1983), J. Appl. Biochem., 5, 235-260.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
glucoamylase I, gel filtration | Coniophora cerebella |
| 48000 | - |
glucoamylase I, approach to equilibrium method, gel filtration | Rhizopus arrhizus |
| 49000 | - |
glucoamylase II, approach to equilibrium method | Mucor rouxianus |
| 54000 | - |
- |
Aspergillus oryzae |
| 54000 | - |
glucoamylase II, approach to equilibrium method, gel filtration | Aspergillus foetidus |
| 55000 | - |
glucoamylase I, gel filtration | Endomyces sp. |
| 59000 | - |
glucoamylase I, approach to equilibrium method | Mucor rouxianus |
| 60000 | - |
x * 60000, glucoamylase II, SDS-PAGE | Aspergillus candidus |
| 60000 | - |
1 * 60000, glucoamylase II, SDS-PAGE | Aspergillus foetidus |
| 62000 | - |
glucoamylase I, gel filtration | Aspergillus phoenicis |
| 67500 | - |
1 * 67500, SDS-PAGE | Aspergillus awamori |
| 69000 | - |
- |
Acremonium charticola |
| 69000 | - |
glucoamylase I, calculation from sedimentation and diffusion data | Aspergillus oryzae |
| 70000 | - |
x * 70000, glucoamylase M2, SDS-PAGE | Aspergillus phoenicis |
| 71600 | - |
gel filtration | Aspergillus awamori |
| 75000 | - |
glucoamylase I, approach to equilibrium method, gel filtration | Aspergillus foetidus |
| 75000 | - |
x * 75000, glucoamylase I, SDS-PAGE | Aspergillus candidus |
| 78000 | - |
1 * 78000, glucoamylase I, SDS-PAGE | Aspergillus foetidus |
| 90000 | - |
x * 90000, glucoamylase I, SDS-PAGE | Aspergillus phoenicis |
| 100000 | - |
glycoamylase I, density gradient ultracentrifugation | Rhizopus arrhizus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acremonium charticola | - |
- |
- |
| Amorphotheca resinae | - |
- |
- |
| Aspergillus awamori | - |
- |
- |
| Aspergillus candidus | - |
- |
- |
| Aspergillus foetidus | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Aspergillus phoenicis | - |
- |
- |
| Coniophora cerebella | - |
- |
- |
| Endomyces sp. | - |
- |
- |
| Mucor rouxianus | - |
- |
- |
| Pyricularia grisea | - |
- |
- |
| Rhizopus arrhizus | - |
- |
- |
| Thermomyces lanuginosus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus oryzae |
| glycoprotein | - |
Mucor rouxianus |
| glycoprotein | - |
Aspergillus awamori |
| glycoprotein | - |
Acremonium charticola |
| glycoprotein | enzyme contains 32 mol of carbohydrate per mol of enzyme | Rhizopus arrhizus |
| glycoprotein | carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage | Aspergillus niger |
| glycoprotein | carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage | Rhizopus arrhizus |
| glycoprotein | carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage | Aspergillus phoenicis |
| glycoprotein | carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage | Aspergillus candidus |
| glycoprotein | carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage | Aspergillus foetidus |
| glycoprotein | glucoamylase I and glucoamylase II contain 53.0 mol of carbohydrate per mol of enzyme | Aspergillus candidus |
| glycoprotein | glucoamylase II contains 40 mol of carbohydrate per mol of enzyme | Aspergillus phoenicis |
| glycoprotein | glucoamylase I contains 52.2 mol of carbohydrate per mol of enzyme, glucoamylase II contains 48.5 mol of carbohydrate per mol of enzyme | Aspergillus foetidus |
| glycoprotein | enzyme contains 89 mol of carbohydrate per mol of enzyme | Rhizopus arrhizus |
| glycoprotein | glucoamylase M1 contains 102 mol of carbohydrate per mol of enzyme, glucoamylase M2 contains 57 mol of carbohydrate per mol of enzyme | Aspergillus phoenicis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-alpha-maltosylglucose + H2O | - |
Aspergillus niger | ? | - |
? |  |
| 4-alpha-nigerosyl-glucose + H2O | - |
Aspergillus niger | ? | - |
? | |
| 6-alpha-maltosylglucose + H2O | - |
Aspergillus niger | ? | - |
? | |
| amylodextrin + H2O | amylodextrin DP-15 | Rhizopus arrhizus | ? | - |
? | |
| amylopectin + H2O | - |
Aspergillus niger | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Rhizopus arrhizus | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Aspergillus oryzae | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Aspergillus phoenicis | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Mucor rouxianus | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Aspergillus awamori | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Acremonium charticola | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Aspergillus niger | glucose + ? | - |
? | |
| amylose + H2O | - |
Rhizopus arrhizus | glucose + ? | - |
? | |
| amylose + H2O | - |
Aspergillus oryzae | glucose + ? | - |
? | |
| amylose + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
| amylose + H2O | - |
Mucor rouxianus | glucose + ? | - |
? | |
| amylose + H2O | - |
Aspergillus awamori | glucose + ? | - |
? | |
| amylose + H2O | - |
Acremonium charticola | glucose + ? | - |
? | |
| dextrin + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
| dextrin + H2O | beta-limit dextrin | Aspergillus niger | ? | - |
? | |
| dextrin + H2O | beta-limit dextrin | Rhizopus arrhizus | ? | - |
? | |
| dextrin + H2O | beta-limit dextrin | Aspergillus oryzae | ? | - |
? | |
| isomaltopentaose + H2O | - |
Aspergillus niger | ? | - |
? | |
| isomaltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
| isomaltose + H2O | - |
Aspergillus niger | glucose | - |
? | |
| isomaltose + H2O | - |
Rhizopus arrhizus | glucose | - |
? | |
| isomaltose + H2O | - |
Aspergillus phoenicis | glucose | - |
? | |
| isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
| isomaltotetraose + H2O | - |
Aspergillus niger | ? | - |
? | |
| isomaltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
| isomaltotriose + H2O | - |
Aspergillus niger | ? | - |
? | |
| isomaltotriose + H2O | - |
Aspergillus awamori | ? | - |
? | |
| maltopentaose + H2O | - |
Aspergillus niger | ? | - |
? | |
| maltopentaose + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
| maltopentaose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| maltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
| maltose + H2O | - |
Aspergillus niger | 2 glucose | - |
? | |
| maltose + H2O | - |
Rhizopus arrhizus | 2 glucose | - |
? | |
| maltose + H2O | - |
Aspergillus oryzae | 2 glucose | - |
? | |
| maltose + H2O | - |
Aspergillus phoenicis | 2 glucose | - |
? | |
| maltose + H2O | - |
Mucor rouxianus | 2 glucose | - |
? | |
| maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? | |
| maltotetraose + H2O | - |
Aspergillus niger | ? | - |
? | |
| maltotetraose + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
| maltotetraose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| maltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
| maltotetraose + H2O | - |
Acremonium charticola | ? | - |
? | |
| maltotriose + H2O | - |
Aspergillus niger | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Rhizopus arrhizus | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Aspergillus oryzae | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Aspergillus phoenicis | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Mucor rouxianus | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Aspergillus awamori | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Acremonium charticola | maltose + glucose | - |
? | |
| methyl-alpha-D-glucoside + H2O | - |
Aspergillus niger | methanol + alpha-D-glucose | - |
? | |
| nigerose + H2O | - |
Aspergillus niger | 2 alpha-D-glucose | - |
? | |
| nigerose + H2O | - |
Aspergillus awamori | 2 alpha-D-glucose | - |
? | |
| panose + H2O | - |
Aspergillus niger | ? | - |
? | |
| panose + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
| panose + H2O | - |
Aspergillus awamori | ? | - |
? | |
| phenyl alpha-glucoside + H2O | - |
Aspergillus niger | ? | - |
? | |
| sucrose + H2O | - |
Aspergillus niger | D-glucose + D-fructose | - |
? | |
| trehalose + H2O | - |
Aspergillus niger | D-glucose | - |
? | |
| turanose + H2O | - |
Aspergillus niger | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Rhizopus arrhizus |
| ? | - |
Mucor rouxianus |
| ? | x * 90000, glucoamylase I, SDS-PAGE | Aspergillus phoenicis |
| ? | x * 75000, glucoamylase I, SDS-PAGE | Aspergillus candidus |
| ? | x * 70000, glucoamylase M2, SDS-PAGE | Aspergillus phoenicis |
| ? | x * 60000, glucoamylase II, SDS-PAGE | Aspergillus candidus |
| monomer | - |
Rhizopus arrhizus |
| monomer | - |
Aspergillus oryzae |
| monomer | - |
Aspergillus candidus |
| monomer | 1 * 78000, glucoamylase I, SDS-PAGE | Aspergillus foetidus |
| monomer | 1 * 67500, SDS-PAGE | Aspergillus awamori |
| monomer | 1 * 60000, glucoamylase II, SDS-PAGE | Aspergillus foetidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Aspergillus oryzae |
| 5 | 5.5 | - |
Acremonium charticola |
| 6.6 | - |
- |
Thermomyces lanuginosus |
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