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Literature summary for 3.2.1.3 extracted from
- Purification and substrate specificity of glucoamylase of Paecilomyces varioti AHU 9417 (1985), Agric. Biol. Chem., 49, 1633-1641.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
soluble starch | soluble | Paecilomyces variotii | |
| 0.03 | - |
amylose | DP = 17 | Paecilomyces variotii |  |
| 0.04 | - |
amylose | DP = 13 | Paecilomyces variotii | |
| 0.11 | - |
maltoheptaose | - |
Paecilomyces variotii | |
| 0.12 | - |
maltohexaose | - |
Paecilomyces variotii | |
| 0.14 | - |
maltotetraose | - |
Paecilomyces variotii | |
| 0.26 | - |
maltotriose | - |
Paecilomyces variotii | |
| 0.31 | - |
Phenyl alpha-maltoside | - |
Paecilomyces variotii | |
| 1.2 | - |
maltose | - |
Paecilomyces variotii | |
| 12 | - |
panose | - |
Paecilomyces variotii | |
| 22 | - |
nigerose | - |
Paecilomyces variotii | |
| 59 | - |
isomaltose | - |
Paecilomyces variotii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
gel filtration | Paecilomyces variotii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paecilomyces variotii | - |
AHU 9417 | - |
| Paecilomyces variotii AHU 9417 | - |
AHU 9417 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paecilomyces variotii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Paecilomyces variotii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Paecilomyces variotii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Paecilomyces variotii | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Paecilomyces variotii AHU 9417 | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Paecilomyces variotii | glucose + ? | - |
? | |
| amylose + H2O | - |
Paecilomyces variotii AHU 9417 | glucose + ? | - |
? | |
| dextrin + H2O | beta-limit dextrin | Paecilomyces variotii | ? | - |
? | |
| dextrin + H2O | beta-limit dextrin | Paecilomyces variotii AHU 9417 | ? | - |
? | |
| glycogen + H2O | - |
Paecilomyces variotii | glucose + ? | - |
? | |
| glycogen + H2O | - |
Paecilomyces variotii AHU 9417 | glucose + ? | - |
? | |
| isomaltose + H2O | - |
Paecilomyces variotii | glucose | - |
? | |
| maltoheptaose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| maltohexaose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| maltopentaose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| maltose + H2O | - |
Paecilomyces variotii | 2 glucose | - |
? | |
| maltotetraose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| maltotriose + H2O | - |
Paecilomyces variotii | maltose + glucose | - |
? | |
| nigerose + H2O | - |
Paecilomyces variotii | 2 alpha-D-glucose | - |
? | |
| panose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| phenyl alpha-maltoside + H2O | - |
Paecilomyces variotii | glucose + phenyl alpha-glucoside | - |
? | |
| soluble starch + H2O | - |
Paecilomyces variotii | beta-D-glucose + ? | - |
? | |
| soluble starch + H2O | - |
Paecilomyces variotii | beta-D-glucose + soluble starch | - |
? | |
| starch + H2O | soluble | Paecilomyces variotii | glucose + ? | beta-glucose | ? | |
| starch + H2O | soluble | Paecilomyces variotii AHU 9417 | glucose + ? | beta-glucose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 69000, SDS-PAGE | Paecilomyces variotii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.4, 15 min, stable up to | Paecilomyces variotii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
isomaltose | - |
Paecilomyces variotii | |
| 2.2 | - |
nigerose | - |
Paecilomyces variotii | |
| 7.2 | - |
maltose | - |
Paecilomyces variotii | |
| 9.1 | - |
panose | - |
Paecilomyces variotii | |
| 11 | - |
Phenyl alpha-maltoside | - |
Paecilomyces variotii | |
| 27.2 | - |
maltotriose | - |
Paecilomyces variotii | |
| 36.5 | - |
amylose | DP = 17 | Paecilomyces variotii | |
| 37.7 | - |
amylose | DP = 13 | Paecilomyces variotii | |
| 41.1 | - |
maltopentaose | - |
Paecilomyces variotii | |
| 41.6 | - |
maltohexaose | - |
Paecilomyces variotii | |
| 43.4 | - |
maltotetraose | - |
Paecilomyces variotii | |
| 46.6 | - |
maltoheptaose | - |
Paecilomyces variotii | |
| 49.1 | - |
soluble starch | - |
Paecilomyces variotii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Paecilomyces variotii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | 4°C, 24 h, stable | Paecilomyces variotii |
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