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Literature summary for 3.2.1.3 extracted from
- Purification and properties of an extracellular glucoamylase from a diastatic strain of Saccharomyces cerevisiae (1988), Biochem. J., 249, 163-170.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.54 | - |
maltose | - |
Saccharomyces cerevisiae |  |
| 3.65 | - |
maltohexaose | - |
Saccharomyces cerevisiae | |
| 3.79 | - |
maltotriose | - |
Saccharomyces cerevisiae | |
| 4.12 | - |
maltotetraose | - |
Saccharomyces cerevisiae | |
| 4.15 | - |
maltoheptaose | - |
Saccharomyces cerevisiae | |
| 4.33 | - |
maltopentaose | - |
Saccharomyces cerevisiae | |
| 36.47 | - |
isomaltose | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
4 * 72000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Rhizopus sp. | - |
- |
- |
| Saccharomyces cerevisiae | - |
1ab | - |
| Saccharomyces cerevisiae 1ab | - |
1ab | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus oryzae |
| glycoprotein | - |
Rhizopus sp. |
| glycoprotein | 80% of the enzyme is carbohydrate | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Saccharomyces cerevisiae | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Aspergillus oryzae | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Rhizopus sp. | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Saccharomyces cerevisiae 1ab | D-glucose + ? | - |
? | |
| dextrin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| dextrin + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| dextrin + H2O | - |
Saccharomyces cerevisiae 1ab | ? | - |
? | |
| glycogen + H2O | - |
Saccharomyces cerevisiae | glucose + ? | - |
? | |
| glycogen + H2O | - |
Aspergillus oryzae | glucose + ? | - |
? | |
| glycogen + H2O | - |
Saccharomyces cerevisiae 1ab | glucose + ? | - |
? | |
| isomaltose + H2O | - |
Saccharomyces cerevisiae | glucose | - |
? | |
| maltoheptaose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| maltoheptaose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| maltohexaose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| maltohexaose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| maltopentaose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| maltopentaose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| maltose + H2O | - |
Saccharomyces cerevisiae | 2 glucose | - |
? | |
| maltose + H2O | - |
Aspergillus oryzae | 2 glucose | - |
? | |
| maltose + H2O | - |
Saccharomyces cerevisiae 1ab | 2 glucose | - |
? | |
| maltotetraose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| maltotetraose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| maltotriose + H2O | - |
Saccharomyces cerevisiae | maltose + glucose | - |
? | |
| maltotriose + H2O | - |
Aspergillus oryzae | maltose + glucose | - |
? | |
| additional information | no hydrolysis of alpha-1,6 linkages | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | no hydrolysis of alpha-1,6 linkages | Saccharomyces cerevisiae 1ab | ? | - |
? | |
| starch + H2O | soluble | Aspergillus oryzae | glucose + ? | - |
? | |
| starch + H2O | soluble | Rhizopus sp. | glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 72000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
half-life: 18.5 min | Rhizopus sp. |
| 60 | - |
- |
Rhizopus sp. |
| 60 | - |
half-life: 26 min | Saccharomyces cerevisiae |
| 60 | - |
half-life: 0.35 min | Aspergillus oryzae |
| 60 | - |
half-life: 88 min | Aspergillus oryzae |
| 65 | - |
half-life: 6 min | Saccharomyces cerevisiae |
| 65 | - |
half-life: 32 min | Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
isomaltose | - |
Saccharomyces cerevisiae | |
| 52 | - |
maltose | - |
Saccharomyces cerevisiae | |
| 328 | - |
glycogen | - |
Saccharomyces cerevisiae | |
| 330 | - |
maltotriose | - |
Saccharomyces cerevisiae | |
| 413 | - |
Dextrin | - |
Saccharomyces cerevisiae | |
| 428 | - |
starch | soluble | Saccharomyces cerevisiae | |
| 683 | - |
amylopectin | - |
Saccharomyces cerevisiae | |
| 717 | - |
maltohexaose | - |
Saccharomyces cerevisiae | |
| 720 | - |
maltotetraose | - |
Saccharomyces cerevisiae | |
| 800 | - |
maltopentaose | - |
Saccharomyces cerevisiae | |
| 823 | - |
maltoheptaose | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | - |
Saccharomyces cerevisiae |
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