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Literature summary for 3.2.1.28 extracted from
- Gene cloning, prokaryotic expression, and biochemical characterization of a soluble trehalase in Helicoverpa armigera Huebner (Lepidoptera Noctuidae) (2018), J. Insect Sci., 18, 22 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Helicoverpa armigera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| castanospermine | effective competitive inhibitor of trehalase, completely inhibited by 0.001 mM | Helicoverpa armigera |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 72.8 | - |
alpha,alpha-trehalose | 55°C, pH 7.4 | Helicoverpa armigera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65610 | - |
calculated from sequence | Helicoverpa armigera |
| 71000 | - |
SDS-PAGE | Helicoverpa armigera |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | Helicoverpa armigera | trehalase is an indispensable component of insect hemolymph that plays important role in energy metabolism and stress resistance | beta-D-glucose + alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicoverpa armigera | A0A0F6PAZ3 | Hübner | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel exchange chromatography | Helicoverpa armigera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | - |
Helicoverpa armigera | beta-D-glucose + alpha-D-glucose | - |
? | |
| alpha,alpha-trehalose + H2O | trehalase is an indispensable component of insect hemolymph that plays important role in energy metabolism and stress resistance | Helicoverpa armigera | beta-D-glucose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71000, SDS-PAGE | Helicoverpa armigera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HaTreh-1 | - |
Helicoverpa armigera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Helicoverpa armigera |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 60 | 37°C: about 60% of maximal activity, 60°C: about 90% of maximal activity | Helicoverpa armigera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Helicoverpa armigera |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | pH 4.0: about 90% of maximal activity, pH 7.0: about 30% of maximal activity | Helicoverpa armigera |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0067 | - |
castanospermine | 55°C, pH 7.4 | Helicoverpa armigera | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Helicoverpa armigera | calculated from sequence | - |
5.02 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | trehalase is an indispensable component of insect hemolymph that plays important role in energy metabolism and stress resistance | Helicoverpa armigera |
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