Cloned (Comment) | Organism |
---|---|
- |
Tenebrio molitor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amygdalin | i.e. glucose-beta-1,6-glucose-beta-mandelonitrile, linear competitive inhibitor | Tenebrio molitor | |
diethyldicarbonate | the compound modifies a His residue that results in a less active enzyme. After 40 min with 40 mM diethylpyrocarbonate at 30°C pH 6.0, trehalase activity decreases to about 50% of the initial activity | Tenebrio molitor | |
glucono-delta-lactone | linear competitive inhibitor | Tenebrio molitor | |
mandelonitrile | mixed-type non-competitive inhibitor | Tenebrio molitor | |
methyl-alpha-D-mannoside | linear competitive inhibitor | Tenebrio molitor | |
additional information | EDTA, polyaminopropyl biguanide, tetranitromethane, N-bromosuccinamide, gentiobiose (glucose-beta-1,6-glucose), methyl alpha-D-glucoside, 1,10-phenanthroline, and Tris (pH 6.0) do not inhibit the trehalase | Tenebrio molitor | |
phloretin | linear competitive inhibitor | Tenebrio molitor | |
phlorizin | i.e. glucose-phloretin, linear competitive inhibitor | Tenebrio molitor | |
prunasin | i.e glucose-beta-mandelonitrile, linear competitive inhibitor | Tenebrio molitor | |
Salicin | linear competitive inhibitor | Tenebrio molitor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
alpha,alpha-trehalose | at pH 6.0 and 30°C | Tenebrio molitor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Tenebrio molitor | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Tenebrio molitor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | Tenebrio molitor | - |
beta-D-glucose + alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tenebrio molitor | P32359 | - |
- |
Purification (Comment) | Organism |
---|---|
Econo-Pac methyl hydrophobic interaction column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration | Tenebrio molitor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelium | - |
Tenebrio molitor | - |
midgut | - |
Tenebrio molitor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.226 | - |
supernatant, at pH 6.0 and 30°C | Tenebrio molitor |
16.67 | - |
after 74fold purification, at pH 6.0 and 30°C | Tenebrio molitor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | - |
Tenebrio molitor | beta-D-glucose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Tenebrio molitor |
Synonyms | Comment | Organism |
---|---|---|
glucose alpha-1,1-glucose hydrolase | - |
Tenebrio molitor |
TRE1 | - |
Tenebrio molitor |
trehalase | - |
Tenebrio molitor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Tenebrio molitor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
phloretin | at pH 6.0 and 30°C | Tenebrio molitor | |
0.043 | - |
methyl-alpha-D-mannoside | at pH 6.0 and 30°C | Tenebrio molitor | |
0.22 | - |
amygdalin | at pH 6.0 and 30°C | Tenebrio molitor | |
0.43 | - |
prunasin | at pH 6.0 and 30°C | Tenebrio molitor | |
0.5 | - |
phlorizin | at pH 6.0 and 30°C | Tenebrio molitor | |
1.4 | - |
glucono-delta-lactone | at pH 6.0 and 30°C | Tenebrio molitor | |
190 | - |
Salicin | at pH 6.0 and 30°C | Tenebrio molitor |