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Literature summary for 3.2.1.28 extracted from

  • Gomez, A.; Cardoso, C.; Genta, F.A.; Terra, W.R.; Ferreira, C.
    Active site characterization and molecular cloning of Tenebrio molitor midgut trehalase and comments on their insect homologs (2013), Insect Biochem. Mol. Biol., 43, 768-780.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Tenebrio molitor

Inhibitors

Inhibitors Comment Organism Structure
amygdalin i.e. glucose-beta-1,6-glucose-beta-mandelonitrile, linear competitive inhibitor Tenebrio molitor
diethyldicarbonate the compound modifies a His residue that results in a less active enzyme. After 40 min with 40 mM diethylpyrocarbonate at 30°C pH 6.0, trehalase activity decreases to about 50% of the initial activity Tenebrio molitor
glucono-delta-lactone linear competitive inhibitor Tenebrio molitor
mandelonitrile mixed-type non-competitive inhibitor Tenebrio molitor
methyl-alpha-D-mannoside linear competitive inhibitor Tenebrio molitor
additional information EDTA, polyaminopropyl biguanide, tetranitromethane, N-bromosuccinamide, gentiobiose (glucose-beta-1,6-glucose), methyl alpha-D-glucoside, 1,10-phenanthroline, and Tris (pH 6.0) do not inhibit the trehalase Tenebrio molitor
phloretin linear competitive inhibitor Tenebrio molitor
phlorizin i.e. glucose-phloretin, linear competitive inhibitor Tenebrio molitor
prunasin i.e glucose-beta-mandelonitrile, linear competitive inhibitor Tenebrio molitor
Salicin linear competitive inhibitor Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
alpha,alpha-trehalose at pH 6.0 and 30°C Tenebrio molitor

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Tenebrio molitor
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Tenebrio molitor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Tenebrio molitor
-
beta-D-glucose + alpha-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Tenebrio molitor P32359
-
-

Purification (Commentary)

Purification (Comment) Organism
Econo-Pac methyl hydrophobic interaction column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration Tenebrio molitor

Source Tissue

Source Tissue Comment Organism Textmining
epithelium
-
Tenebrio molitor
-
midgut
-
Tenebrio molitor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.226
-
supernatant, at pH 6.0 and 30°C Tenebrio molitor
16.67
-
after 74fold purification, at pH 6.0 and 30°C Tenebrio molitor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
Tenebrio molitor beta-D-glucose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Tenebrio molitor

Synonyms

Synonyms Comment Organism
glucose alpha-1,1-glucose hydrolase
-
Tenebrio molitor
TRE1
-
Tenebrio molitor
trehalase
-
Tenebrio molitor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
-
Tenebrio molitor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
phloretin at pH 6.0 and 30°C Tenebrio molitor
0.043
-
methyl-alpha-D-mannoside at pH 6.0 and 30°C Tenebrio molitor
0.22
-
amygdalin at pH 6.0 and 30°C Tenebrio molitor
0.43
-
prunasin at pH 6.0 and 30°C Tenebrio molitor
0.5
-
phlorizin at pH 6.0 and 30°C Tenebrio molitor
1.4
-
glucono-delta-lactone at pH 6.0 and 30°C Tenebrio molitor
190
-
Salicin at pH 6.0 and 30°C Tenebrio molitor