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Literature summary for 3.2.1.28 extracted from

  • Gu, J.; Shao, Y.; Zhang, C.; Liu, Z.; Zhang, Y.
    Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens (2009), J. Insect Physiol., 55, 997-1002.
    View publication on PubMed
Search for more literature for 3.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, analysis, and comparisons Nilaparvata lugens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane NlTre2, contains a putative transmembrane domain Nilaparvata lugens 16020
-
additional information NlTre-1 contains trehalase signature motifs Nilaparvata lugens
-
-
additional information NlTre-2 contains trehalase signature motifs Nilaparvata lugens
-
-
soluble NlTre1, the soluble trehalase isozyme accounts for the majority of total trehalase activity Nilaparvata lugens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trehalose + H2O Nilaparvata lugens
-
 2 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Nilaparvata lugens C0LZJ6 NlTre-1; brown planthopper, gene NlTre-1 encoding the soluble isozyme
-
Nilaparvata lugens C0LZJ7 NlTre-2; brown planthopper, gene NlTre-2 encoding the membrane-bound isozyme
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information activity and gene mRNA level at different developmental stages, expression pattern, overview Nilaparvata lugens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.2
-
 membrane-bound isozyme Nilaparvata lugens
39.4
-
 soluble isozyme Nilaparvata lugens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
 Nilaparvata lugens 2 D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
trehalase
-
 Nilaparvata lugens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nilaparvata lugens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Nilaparvata lugens

Expression

Organism Comment Expression
Nilaparvata lugens no induction by 20-hydroxyecdysone of the membrane-bound trehalase isozyme encoded by gene NlTre2 additional information
Nilaparvata lugens 20-hydroxyecdysone induces the soluble trehalase isozyme encoded by gene NlTre1 up

General Information

General Information Comment Organism
physiological function the enzyme trehalase is involved in energy metabolism and controlling trehalose, the main blood sugar of insects, levels in cells Nilaparvata lugens