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Literature summary for 3.2.1.28 extracted from

  • Liu, Y.; Wang, Z.; Yin, Y.; Cao, Y.; Zhao, H.; Xia, Y.
    Expression, purification, and characterization of recombinant Metarhizium anisopliae acid trehalase in Pichia pastoris (2007), Protein Expr. Purif., 54, 66-72.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain KM71 Metarhizium anisopliae

Inhibitors

Inhibitors Comment Organism Structure
trehazolin specific inhibitor Metarhizium anisopliae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
trehalose native enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C Metarhizium anisopliae
2.6
-
trehalose recombinant enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C Metarhizium anisopliae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
SDS-PAGE, recombinant enzyme Metarhizium anisopliae

Organism

Organism UniProt Comment Textmining
Metarhizium anisopliae A9XE63 fragment
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Metarhizium anisopliae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101.3
-
recombinant enzyme, after purification, at 30°C Metarhizium anisopliae
120
-
native enzyme, after purification, at 30°C Metarhizium anisopliae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O high specificity for trehalose Metarhizium anisopliae D-glucose
-
?

Synonyms

Synonyms Comment Organism
acid trehalase
-
Metarhizium anisopliae
ATM1 mature enzyme Metarhizium anisopliae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant enzyme Metarhizium anisopliae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 recombinant enzyme Metarhizium anisopliae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme is stable below 50°C, but it loses activity rapidly above 50°C Metarhizium anisopliae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
recombinant enzyme Metarhizium anisopliae

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 recombinant enzyme Metarhizium anisopliae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8 after incubation at pH values of 3.5-8.0 and 30°C for 1 h, all residual activities of reATM1 are over 80% Metarhizium anisopliae

pI Value

Organism Comment pI Value Maximum pI Value
Metarhizium anisopliae native enzyme, isoelectric focusing
-
4.7
Metarhizium anisopliae recombinant enzyme, isoelectric focusing
-
4.9

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000013
-
native enzyme, in 20 mM MES, pH 6.0, at 30°C Metarhizium anisopliae trehazolin
0.000019
-
recombinant enzyme, in 20 mM MES, pH 6.0, at 30°C Metarhizium anisopliae trehazolin